African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12179

Full Length Research Paper

Extracellular biogenic synthesis of silver nanoparticles by Actinomycetes from amazonic biome and its antimicrobial efficiency

Nélly Mara Silva-Vinhote
  • Nélly Mara Silva-Vinhote
  • Department of Parasitology, Institute of Biological Sciences, Federal University of Amazonas, UFAM, Manaus, Amazonas, AM, Brazil.
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Nelson Eduardo Durán Caballero
  • Nelson Eduardo Durán Caballero
  • Chemistry Institute, University of Campinas, UNICAMP, Campinas, SP, Brazil.
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Taciana de Amorim Silva
  • Taciana de Amorim Silva
  • Department of Parasitology, Institute of Biological Sciences, Federal University of Amazonas, UFAM, Manaus, Amazonas, AM, Brazil.
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Patrick Veras Quelemes
  • Patrick Veras Quelemes
  • Research Centre in Biodiversity and Biotechnology, Campus Ministro Reis Velloso, Federal University of Piauí, UFPI, Parnaíba, PI, Brazil.
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Alyne Rodrigues de Araújo
  • Alyne Rodrigues de Araújo
  • Research Centre in Biodiversity and Biotechnology, Campus Ministro Reis Velloso, Federal University of Piauí, UFPI, Parnaíba, PI, Brazil.
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Ana Carolina Mazarin de Moraes
  • Ana Carolina Mazarin de Moraes
  • Chemistry Institute, University of Campinas, UNICAMP, Campinas, SP, Brazil.
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Ana Lygia dos Santos Câmara
  • Ana Lygia dos Santos Câmara
  • Department of Genetics and Morphology, Institute of Biological Sciences, University of Brasília, BSB, Brazil.
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João Paulo Figueiró Longo
  • João Paulo Figueiró Longo
  • Department of Genetics and Morphology, Institute of Biological Sciences, University of Brasília, BSB, Brazil.
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Ricardo Bentes Azevedo
  • Ricardo Bentes Azevedo
  • Department of Genetics and Morphology, Institute of Biological Sciences, University of Brasília, BSB, Brazil.
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Durcilene Alves da Silva
  • Durcilene Alves da Silva
  • Research Centre in Biodiversity and Biotechnology, Campus Ministro Reis Velloso, Federal University of Piauí, UFPI, Parnaíba, PI, Brazil.
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José Roberto de Souza de Almeida Leite
  • José Roberto de Souza de Almeida Leite
  • Research Centre in Biodiversity and Biotechnology, Campus Ministro Reis Velloso, Federal University of Piauí, UFPI, Parnaíba, PI, Brazil.
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Maria Francisca Simas Teixeira
  • Maria Francisca Simas Teixeira
  • Department of Parasitology, Institute of Biological Sciences, Federal University of Amazonas, UFAM, Manaus, Amazonas, AM, Brazil.
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  •  Received: 12 July 2017
  •  Accepted: 25 September 2017
  •  Published: 25 October 2017

Abstract

The microbial sources used for the production of nanoparticles are important, and one of the reasons for bacterial preference is their ease of manipulation and ecofriendly potential. Three bacteria strains of Streptomyces genus were used for the biosynthesis of silver nanoparticles (AgNPs). The aim of the present study was to confirm Streptomyces spp. as biological systems in the synthesis and extracellular characterization of silver nanoparticles with antimicrobial activity. The nanoparticles were characterized by ultraviolet–visible spectroscopy (UV-Vis), dynamic light scattering (DLS), RDX, Fourier-transform infrared spectroscopy (FTIR) and transmission electron microscope (TEM). In qualitative antimicrobial test, Streptomyces parvulus DPUA 1549 and Streptomyces owasiensis DPUA 1748 showed activity against pathogenic microorganisms (bacteria and yeasts) tested. The AgNPs of S. parvulus DPUA 1549 showed the most efficient minimum inhibitory concentration (MIC) at low concentrations (1.95 μM) against Staphylococcus aureus. They promoted structural damage to the micro-organism cell surface according to AFM images and also reduced the proliferation of NIH-3T3 cells in the cytotoxic assay. Thus, biosynthesis of silver nanoparticles by Streptomyces spp. is a potential source to be used in nanomedicine and cosmetology industries.

 

Key words: Streptomyces, tropical region, nanobiotechnology, antimicrobial, cytotoxic test.