African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12486

Full Length Research Paper

Isolation and partial characterization of a protease enzyme from Thaumatococcus daniellii waste

Raimi, O. G.1,2*, Elemo, B. O.1, Fatai, A. A.1, Bankole, H. A.1, Kazeem, M. I.1and Banjoko, A. O.1
1Department of Biochemistry, Lagos State University, Ojo, P. O. Box 0001, LASU Post Office, Lagos, Lagos State, Nigeria. 2School of Life Sciences, University of Dundee, Scotland, United Kingdom.
Email: [email protected]

  •  Accepted: 18 January 2011
  •  Published: 11 April 2011

Abstract

A protease enzyme was isolated and partially purified from the pulp ofThaumatococcus daniellii fruit by gel filtration on sephadex G-75 followed by ion-exchange column chromatography on DEAE-cellulose. The enzyme showed a specific activity of 4.75 × 10-1 unit/mg protein and 6.93 × 10-1 unit/mg protein, respectively after each purification procedure. The purified enzyme had a Km andVmax of 2.0 × 10-4 M and 1.53 mol/min, respectively, using casein as substrate.  The enzyme had an optimum temperature of 35°C and functioned best at pH 7.0 with some residual activity at alkaline pH.

 

Key words: Protease, isolation, characterization, Thaumatococcus danielii.

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This article is published under the terms of the Creative Commons Attribution License 4.0

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