African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12487

Full Length Research Paper

Identification of differentially expressed genes from male and female flowers of kiwifruit

Ho Bang Kim1*, Sung-Soo Jun2, Sunghwa Choe3, Jae Youl Cho4, Sang-Bong Choi5 and Seong-Cheol Kim6*
1The Natural Science Research Institute, Myongji University, Yongin 449-728, Republic of Korea. 2School of General Education, Institute for Scientifically Able Youth, Kyungwon University, Seoungnam 461-701, Republic of Korea. 3School of Biological Sciences, College of Natural Sciences, Seoul National University, Seoul 151-747, Republic of Korea. 4School of Bioscience and Biotechnology, and Institute of Bioscience and Biotechnology, Kangwon National University, Chuncheon 200-701, Republic of Korea. 5Department of Biological Sciences, Myongji University, Yongin 449-728, Republic of Korea. 6Agricultural Research Center for Climate Change, National Institute of Horticultural and Herbal Science, Rural Development Administration, Jeju 690-150, Republic of Korea.
Email: [email protected], [email protected]

  •  Accepted: 23 March 2010
  •  Published: 04 October 2010

Abstract

Kiwifruit is one of the dioecious plant species, whereas most flowering plants are hermaphrodite. Sex chromosome-linked genes have been isolated mainly from the dioecious plant Silene latifolia. As an attempt to understand the molecular mechanisms of sex determination and differentiation in kiwifruit, we performed DEG (differentially expressed gene) screening using annealing control primer-based gene fishing method from male and female floral buds. As a result, 15 DEGs in total were isolated and then sequenced. RT-PCR analysis revealed that some of them were differentially expressed in either male or female floral buds. GenBank database searches revealed that 11 DEGs exhibited significant sequence similarity with known function of genes or ESTs of kiwifruit. Among them, kiwiDEG1 encodes partial open reading frame (ORF) for pectin methylesterase, which plays an important role in pollen development as a cell-wall modifying enzyme. It was abundantly expressed in male floral buds compared with those of female, suggesting its functional role in pollen development of kiwifruit. KiwiDEG9 encodes a full-length ORF for adenosine diphosphate (ADP)-ribosylation factor, which is a small guanosine-5'-triphosphate (GTP)-binding protein and plays an essential role in vesicle trafficking. Its expression level was higher in male floral buds than in female. The isolated kiwiDEGs will be useful resources to better understand the molecular events of sex differentiation in kiwifruit.

 

Key words: Actinidia, differentially expressed genes, dioecious plant, male flowers, female flowers, kiwifruit, pectin methylesterase, ADP-ribosylation factor.

Abbreviation

DEG, Differentially expressed gene; ORF, open reading frame; STM, shootmeristemless; CUC1, cup shaped cotyledon 1; RT-PCR, reverse transcription-mediated polymerase chain reaction; ESTs, expressed sequence tags; PME, pectin methylesterase; PGA, polygalacturonic acid; SP, signal peptide;TM, transmembrane domain; PMEI, pectin methylesterase inhibitor; PM,processing motif; ARFs, adenosine diphosphate-ribosylation factors; ARL, ARF-like proteins; GEFs, guanine nucleotide exchange factors; FADs, fatty acid desaturases; LEA, late embryogenesis abundant.

Copyright © 2024 Author(s) retain the copyright of this article.

This article is published under the terms of the Creative Commons Attribution License 4.0

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