African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12487

Full Length Research Paper

Diagnosis and surveillance of rinderpest using reverse transcription - PCR

E. Couacy-Hymann1*, S.C. Bodjo1,2, T. Danho1, M.Y. Koffi1, C. Akoua-Koffi3
1LANADA / Laboratoire Central de Pathologie Animale de Bingervill e, BP 206 Bingerville, Côte-d’Ivoire. 3Institut Pasteur , Département Virologie – Epidémiologie – 01 BP 490 Abidjan 01, Côte-d’Ivoire. 2Animal Production Unit, FAO / IAEA Agriculture and Biotechnology Laboratory, Laboratories, A-2444 Seibersdorf, Austria.
Email: [email protected]

  •  Accepted: 23 June 2006
  •  Published: 02 October 2006

Abstract

PCR technique was used as an alternative method to detect evidence of rinderpest virus for diagnosis and in epidemiological surveys. Viral RNA was purified in 20 to 30 min using a commercial kit (RNaid (BIO 101). Primers used mapped in the nucleocapsid protein gene of rinderpest virus and gave specific and sensitive amplification from pathological samples. The size of the amplified fragment was 297 bp and the result was confirmed using internal non-radioactive probe SB1. The specificity of the PCR products was also confirmed by cleavage using restriction enzyme RsaI to give a major band of 200 bp.

 

Key words: RPV, RT-PCR, rinderpest, PPRV, morbillivirus.

Copyright © 2024 Author(s) retain the copyright of this article.

This article is published under the terms of the Creative Commons Attribution License 4.0

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