Abstract

Morphological and anatomical evaluation on three varieties of Vitis vinifera (Trerash, Rash meri and Baidhaw) commonly known as grape vine was reported. The samples were prepared in a mixture containing 95% of alcohol and glacial acetic acid. The result revealed differences among the varieties ranging from morphological to aesthetic features of the leaf. Although, starch grains, druses and raphid crystals were present in all the varieties, the shape of the stem, petiole, midrib, lamina and margin were anatomically different as well as the presence of trichomes among the varieties. The microstructure of the leaf epidermis was investigated using light microscope (LM) and scanning electron microscope (SEM) which confirmed the polygonal shape of the leaf epidermal cells and the anticlinal walls which were straight and arched from the surface view. The structure of the stomata apparatus was present only in the abaxial epidermis and anomocyte type. SEM was used to investigate the pollen grains tricolporat and sub prolate often known as prolate-spheroidal. The flower was bisexual having size ranging between 4 to 5 mm.

Key words: Vitis vinifera, morphological and anatomical evaluation, light microscope, SEM.

Abbreviation

LM, Light microscope; SEM, scanning electron microscope; PBS,phosphate buffer solution; LM, light microscope.