African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12488

Full Length Research Paper

Fermentation process for alcoholic beverage production from mahua (Madhuca indica J. F. Mel.) flowers

Rupesh Singh1, B. K. Mishra2*, K. B. Shukla3, N. K. Jain4, K. C. Sharma4, Sunil Kumar5, Krishna Kant2 and J. K. Ranjan2
1Department of Molecular Biology and Biotechnology, RCA, MPUAT, Udaipur, India. 2NRC on Seed Spices, Ajmer, India. 3Department of Plant Physiology, RCA, MPUAT, Udaipur, India. 4AICRP on Post Harvest Technology, CTAE, MPUAT, Udaipur, India. 5CIPHET, Abohar, Punjab, India.  
Email: [email protected]

  •  Accepted: 23 August 2013
  •  Published: 25 September 2013

Abstract

Mahua flowers are rich in sugar (68-72%), in addition to a number of minerals and one of the most important raw materials for alcohol fermentation. The present investigation was for the development of a non-distilled alcoholic beverage from Mahua flowers. Eighteen (18) treatment combinations consisting of two temperatures (25 and 30°C), three pH (4.0, 4.5 and 5.0) and three period of fermentation (7, 14 and 21 days) were used in the fermentation conditions. The maximum yield of ethanol (9.51 %) occurred at 25°C with pH 4.5 after 14 days of fermentation of Mahua flower juice. The fermented non-distilled alcoholic beverage contained total sugar (8.83 mg/ml), reducing sugar (0.82 mg/ml), total soluble solids (6.37°Brix) titrable acidity (0.65 %), and volatile acidity (0.086%). Methanol was not detected at any stage of fermentation. The developed fermented alcoholic beverage had characteristic flavor and aroma of Mahua flowers with about 7 to 9% alcohol.

 

Key words:  Madhuca indica, ethanol, reducing sugar, fermentation.