African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12193

Full Length Research Paper

In vitro plant regeneration from Narbon Vetch (Vicia narbonensis L.) using cotyledonary node explants

Hayrettin Kendir, Nurdan Sahin-Demirbag, Khalid Mahmood Khawar*, and Muhammad Aasim
Department of Field Crops, Faculty of Agriculture, Ankara University Ankara, 06110, Diskapi, Turkey.
Email: [email protected]

  •  Accepted: 18 June 2008
  •  Published: 18 July 2008

Abstract

 

Narbon vetch (Vicia narbonensis L.) is an agriculturally important forage plant that widely grows in an area extending from Central Europe to various parts of Asia. The study reports axillary shoot regeneration from cotyledonary node explants obtained from, in vitro raised seeds of 4 - 5 and 14 - 15 days old seedlings on MS medium containing 2 - 6 mg/l kinetin-0.1 mg/l indole-3-butyric acid (IBA). No shoot regeneration was recorded on 14 - 15 days old cotyledon node explants. Whereas, 4 - 5 days old cotyledon node explants showed high regeneration potential with the highest number of 3.85 shoots per explant, with mean shoot length of 2.11 cm and shoot regeneration frequency of 93.33%. The shoots obtained from all regeneration media could be easily rooted after pulse treatment with 50 mg/l IBA for 7 min. All of which were morphologically normal and fertile and easily established under greenhouse conditions.

 

Key words: Narbon vetch, Vicia narbonensis L, axillary shoot regeneration, rooting.

Abbreviation

 BAP, 6 Benzylaminopurine; NAA, α Naphthalene acetic acid; IBA, Indole 3 butyric acid; TDZ, Thidiazuron