African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12487

Full Length Research Paper

Screening model for indolocarbazole-producing microorganisms by molecular probe combined with high performance liquid chromatography (HPLC) method

Zhenping Shi
  • Zhenping Shi
  • Qingdao University of Science and Technology, Qingdao 266042, China.
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Jiaojiao Dong
  • Jiaojiao Dong
  • First Institute of Oceanography, State Oceanic Administration, Qingdao 266061, China.
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Wenhan Lin
  • Wenhan Lin
  • State Key Laboratory of Natural and Biomimetic Drugs, Peking University, Beijing100083, China.
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Aigang Yang
  • Aigang Yang
  • Qingdao University of Science and Technology, Qingdao 266042, China.
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Jiuming Zhang
  • Jiuming Zhang
  • Qingdao University of Science and Technology, Qingdao 266042, China.
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Li Tian*
  • Li Tian*
  • Qingdao University of Science and Technology, Qingdao 266042, China.,First Institute of Oceanography, State Oceanic Administration, Qingdao 266061, China.
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  •  Received: 14 June 2013
  •  Accepted: 23 July 2013
  •  Published: 07 January 2015

Abstract

Indolocarbazole (ICZ) alkaloid constitutes a group of natural products which manifested powerful biological activity, especially anticancer. However, it was laborious to find ICZs-yield organism when anticancer activity was adopted as selection target. The specificity of 6 primer pairs was detected and the results revealed that primer rebBN1/rebBC1 possessed preferable specificity because indolocarbazole-producers DNA was exclusively amplified and no target fragment was amplified from any of indolocarbazole nonproducers. Therefore, primer rebBN1/rebBC1 could specifically identify indolocarbazole-producers from microorganism strains. 
 
Key words: Indolocarbazole, rebBN1/rebBC1, ICZs-producing microorganism screening.
 

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