African Journal of

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12392

Full Length Research Paper

Evaluation of Curcuma zerumbet (Zingibaraceae) rhizome extracts sub-acute toxicity on Wistar rats

Márcia Seixas de Castro
  • Márcia Seixas de Castro
  • Federal University of Amazonas/Post-graduation Program in Biotechnology, General Rodrigo Octávio Avenue, 6200, Coroado 1, CEP 69080-900, Manaus-AM, Brazil.
  • Google Scholar
Carlos Cleomir de Souza Pinheiro
  • Carlos Cleomir de Souza Pinheiro
  • National Research Institute of the Amazon/Coordination of Research in Innovation and Technology, Manaus-AM, Brazil.
  • Google Scholar
Helyde Albuquerque Marinho
  • Helyde Albuquerque Marinho
  • National Institute of Research of the Amazon/Coordination of Research in Health Sciences, Manaus-AM, Brazil.
  • Google Scholar
Amadis Batista Francisco
  • Amadis Batista Francisco
  • University of the State of Amazonas/Post-Graduation Program Bionorte Network. Av. Carvalho Leal-S/N-Manaus-AM, Brazil.
  • Google Scholar

  •  Received: 17 January 2018
  •  Accepted: 20 July 2018
  •  Published: 25 July 2018


Plant extracts have been lately used by the population to treat various types of diseases, and this has been notably encouraged by the World Health Organization (WHO). Curcuma zerumbet (Zingiberaceae) belonging to the family of the Zingiberaceae, is herbaceous, perennial and, utilized by the population to treat gastric disorders. However, data on the subacute toxicity of this species are scarce in the literature. Therefore, the present work aimed to ascertain the subacute toxicity of different doses of the aqueous and hydroalcoholic extracts from C. zerumbet. These extracts were orally administered through gavage in Wistar rats for 28 consecutive days. This study followed the instructions put forth by Guideline 407 (subacute toxicity) of Organization for Economic Cooperation and Development (OECD). In specific pharmacological tests for acute and subacute toxicity in rats, it can be stated that C. zerumbet extracts doses greater than 5 g/kg neither caused mortality nor presented oral toxicity. Therefore, the extracts toxicity parameters analyzed in different doses on the groups of animals have shown no significant difference from those found in the control group. This allows one to conclude C. zerumbet (Zingiberaceae) rhizome aqueous and alcoholic extracts in high doses to harbor very low toxicity within a short time.


Key words: Curcuma, subacute toxicity, extracts.