African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12487

Full Length Research Paper

Effect of acid hydrolysis of Garcinia kola (bitter kola) pulp waste on the production of CM-cellulase and β- glucosidase using Aspergillus niger

Caritas U. Okafoagu1 and Humphrey C. Nzelibe2*
National Agency for Food and Drugs Administration and Control, Kaduna Area  Laboratory, Kaduna,  Nigeria. Department of Biochemistry, Ahmadu Bello University, Zaria, Nigeria.  
Email: [email protected]

  •  Accepted: 26 February 2006
  •  Published: 31 May 2006

Abstract

Effect of acid hydrolysis of bitter kola (Garcinia kola) pulp wastes on the production of CM-cellulase and β-glucosidase using Aspergillus niger was investigated. Hydrolysis for 3 h with 2.5 M sulphuric acid yielded highest fermentable sugar. Acid hydrolysis enhanced CM-cellulase and β-glucosidase levels by 500% at 96 h and 200% at 120 h, respectively. Acid hydrolysed G. kola pulp wastes is a better substrate for the production of CM-cellulase and β-glucosidase compared with maize and rice husks.
 
Key words: Bitter kola pulp wastes, CM-cellulase, β-glucosidase, Aspergillus niger, acid hydrolysis.

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This article is published under the terms of the Creative Commons Attribution License 4.0

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