African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12227

Full Length Research Paper

Analysis of Kenyan isolates of Fusarium solani f. sp. phaseoli from common bean using colony characteristics, pathogenicity and microsatellite DNA

Agnes W. Mwang’ombe1*, Pixley K. Kipsumbai1, Ezekiel K. Kiprop2, Florence M. Olubayo1 and Joel W. Ochieng3,4
  1Department of Plant Science and Crop Protection, Faculty of Agriculture, University of Nairobi, P.O Box 30197-00100, Nairobi, Kenya. 2Department of Biological Sciences, School of Science, Moi University, P.O. Box 1125-30100, Eldoret, Kenya. 3Faculties of Agriculture and Veterinary Medicine, University of Nairobi, Kenya.  4Centre for Plant Conservation Genetics, Southern Cross University, P.O. Box 157 Lismore NSW, 2480 Australia.
Email: [email protected]

  •  Accepted: 24 January 2008
  •  Published: 03 June 2008

Abstract

 

Fusarium solani (Mart) f.sp. phaseoli (Burk) Synd. and Hans., is a plant pathogenic fungus that causes root rot in garden bean (Phaseolus vulgaris L.). To evaluate methods used in classifying strains of this pathogen, 52 Fusarium solani f.sp. phaseoli isolates from infected bean plants grown on different farms in Taita hills of Coast province, Kenya, were cultured and characterized using morphology, pathogenicity and microsatellite DNA. All the isolates showed high variability in aerial mycelial growth, mycelia texture, pigmentation (mycelia colour) when cultured on potato dextrose agar medium, and conidial measurements onSpezieller Nahrstoffarmer agar medium. Colonies were grouped into luxuriant, moderately luxuriant and scanty on aerial mycelial growth; fluffy and fibrous based on mycelial texture; purple, pink and white based on mycelia colour; and long, medium and short macroconidial length. All the isolates were pathogenic on GLP-2 (Rosecoco), a susceptible bean variety commonly grown in Kenya. DNA analysis showed that the isolates carried a high genetic diversity (gene diversity = 0.686; mean number of alleles = 9). Neighbour-Joining phylogenetic clusters reconstructed using microsatellite variation showed three major clusters. However, the microsatellite groupings were independent of the altitude, colony characteristics and virulence of the isolates.

 

Key words: Fusarium solani, phaseoli, fungus, microsatellite, genetic diversity,Phaseolus vulgaris.