African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12275

Full Length Research Paper

Physicochemical properties of rhodanese: A cyanide detoxifying enzyme from Pentadiplandra brazzeana (Baill) root

Raphael E. Okonji
  • Raphael E. Okonji
  • Department of Biochemistry, Faculty of Science, Obafemi Awolowo University, Ile-Ife, Nigeria.
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Bamidele S. Fagbohunka
  • Bamidele S. Fagbohunka
  • Department of Biochemistry, Faculty of Basic Medical Science, Olabisi Onabanjo University, Ago-Iwoye, Nigeria.
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Leonard O. Ehigie
  • Leonard O. Ehigie
  • Department of Biochemistry, Faculty of Basic Medical Science, Ladoke Akintola University, Ogbomoso, Nigeria.
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Zainab A. Ayinla
  • Zainab A. Ayinla
  • Department of Biochemistry, Faculty of Science, Obafemi Awolowo University, Ile-Ife, Nigeria.
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Olajumoke O. Ojo
  • Olajumoke O. Ojo
  • Department of Biochemistry, Faculty of Science, Ekiti State University, Ado-Ekiti, Nigeria.
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  •  Received: 18 November 2016
  •  Accepted: 06 February 2017
  •  Published: 05 April 2017

Abstract

Rhodanese a ubiquitous sulphur transferase enzyme catalyses the detoxification of cyanide to a less toxic thiocyanate. Rhodanese from the root of Pentadiplandra brazzeana was purified by ammonium sulphate precipitation and affinity chromatography. The enzyme had a specific activity of 4.82 RU/mg of protein. The Km values of the substrates (KCN and Na2S2O3) were 11.76 and 10 mM, respectively. The optimum pH and temperature of the enzyme activity were 8.0 and 60°C, respectively. The enzyme was not inhibited by the heavy metals (BaCl2, KCl, NiCl2, NaCl, MnCl2, and ZnCl2). This study affirmed the presence of rhodanese in the root of P. brazzeana, an indication that the plant may be of useful biotechnological application, especially in bioremediation of cyanide intoxified farm sites.

 

Key words: Cyanide, rhodanese, detoxification, inhibition, Pentadiplandra brazzeana.