African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12293

Table of Content: 5 April, 2017; 16(14)

April 2017

Biosynthesis of silver nanoparticles by endophytic fungi: Its mechanism, characterization techniques and antimicrobial potential

Nanotechnology has rapidly developed as an important field of modern research, generating most promising applications in electronics and medicine. It involves different approaches for the synthesis and application of nanoparticles having dimension smaller than 100 nm. Nanoparticles are the fundamental building blocks for preparing many nanostructured materials and devices. Therefore, there is an enormous interest in...

Author(s): Sardul Singh Sandhu, Harshita Shukla and Shyamji Shukla

April 2017

Effects of solid matrix priming with Trichoderma harzianum on seed germination, seedling emergence and photosynthetic capacity of eggplant

The effects of solid matrix priming with strain T22 of Trichoderma harzianum on seed germination, seedling emergence and photosynthetic capacity in two eggplant varieties were evaluated. Seed were primed by mixing with vermiculite and the T. harzianum wettable powder (WP) suspension at a rate of 1:3:3.2 (fresh weigh basis, respectively) at 15°C for 7 days in the dark, then primed seed were dried. Solid matrix...

Author(s): Lingyun WU, Dongwei YAO and Ming LI

April 2017

Physicochemical properties of rhodanese: A cyanide detoxifying enzyme from Pentadiplandra brazzeana (Baill) root

Rhodanese a ubiquitous sulphur transferase enzyme catalyses the detoxification of cyanide to a less toxic thiocyanate. Rhodanese from the root of Pentadiplandra brazzeana was purified by ammonium sulphate precipitation and affinity chromatography. The enzyme had a specific activity of 4.82 RU/mg of protein. The Km values of the substrates (KCN and Na2S2O3) were 11.76 and 10 mM, respectively. The optimum pH and...

Author(s): Raphael E. Okonji, Bamidele S. Fagbohunka, Leonard O. Ehigie, Zainab A. Ayinla and Olajumoke O. Ojo

April 2017

Quality characteristics of Korean traditional rice wine (Takju) prepared by addition of green pepper

Makgeolli, frequently called Takju, a famous Korean traditional rice wine, has long been prepared and consumed in Korea. Takju consumption in Korea has been increased because of its nutritional and functional values. The objective of the present study was to investigate the chemical characteristics, color values, 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activities, total phenolic contents, and sensory...

Author(s): Chan Yong Kim, Kil Su Jang, Su Gyeong Jeon, Sanjeev Kumar Dhungana, Hyeon Min Do, Oh Hun Kwon, Oh-Heun Kwon, Hye-Ryun Kim and Il-Doo Kim

April 2017

Evaluation of the role of SsaV ‘Salmonella pathogenicity island-2 dependent type III secretion system components on the virulence behavior of Salmonella enterica serovar Typhimurium

Salmonella is a causative agent of wide range of diseases varying from gastroenteritis to systemic typhoid fever. It uses specialized Type III secretion system (T3SS) by its two compartments to invade and intracellularly survive inside the immune cells. T3SS is expressed in two subsequent phases by two distinct Salmonella pathogenicity islands (SP) I and II. Understanding and evaluation of components T3SS-SPI1 and...

Author(s): Wael A. H. Hegazy, and Hisham A. Abbas

April 2017

Assessment of genetic diversity and structure of Sporisorium scitamineum from China using inter-simple sequence repeat (ISSR) markers

In this study, the genetic diversity and structure of 90 monosporidia mating-type isolates of Sporisorium scitamineum collected from China were comprehensively evaluated based on the DNA extracted from mating-type haploid sporidias using inter-simple sequence repeat (ISSR) polymorphism molecular marker technique. Fifteen selected ISSR primers produced 162 amplified DNA fragments, of which 115 (71.0%) were polymorphic....

Author(s): Gang-hong Xu, Quan-qing Deng, Wan-kuan Shen,, Shuang Chen, and Xia-ming Wu,

April 2017

First detection of deformed wing and kakugo viruses in honeybees (Apis mellifera L.) in Egypt by real time-polymerase chain reaction (RT-PCR)

The identification of bee viruses is of great importance where there is no information on their natural occurrence in honeybee populations. Honeybee samples (Apis mellifera L.) obtained from Giza Governorate, Egypt were evaluated for total RNA from the head, thorax and abdomen, and varroa mites. Two fragments of 250 and 540 bp of deformed wing (DWV) and kakugo viruses (KV), respectively, were amplified using...

Author(s): Emtithal M. Abd-El-Samie, Fatma K. Adham, Sawsan El-Mohandes and Heba Seyam

April 2017

Micropropagation of two species of Micranthocereus (Cactaceae) with ornamental potential native to Bahia, Brazil

The two target cactus species in this study, Micranthocereus flaviflorus subsp. densiflorus (Buining and Brederoo) P.J.Braun and Esteves and Micranthocereus. polyanthus subsp. alvinii M. Machado and Hofacker, are endemic to the state of Bahia and have ornamental value. Therefore, this work proposed to establish M. flaviflorus in vitro, as well as to micropropagate both species. The temperature regimes 25°C and...

Author(s): Laila Mandel Civatti, Maria Nazaré Guimarães Marchi and Moema Cortizo Bellintani