African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12081

Full Length Research Paper

Genetic diversity of male fig (Ficus carica caprificus L.) genotypes with random amplified polymorphic DNA (RAPD) markers

Zeynel Dalkılıç1, H. Osman Mestav1,2, Gonca Günver-Dalkılıç1 and Hilmi Kocataş3
  1Department of Horticulture, Faculty of Agriculture, Adnan Menderes University, South Campus, 09100 Aydın, Turkey. 2Çine Vocational School, Adnan Menderes University, 09500 Aydın, Turkey. 3Erbeyli Fig Research Institute, 09600 Aydın, Turkey.
Email: [email protected]

  •  Accepted: 18 November 2010
  •  Published: 24 January 2011

Abstract

 

The objective of this research is to characterize and relate 43 different male fig (Ficus carica caprificus L.) genotypes grown in Aydın Province, Turkey using random amplified polymorphic DNA (RAPD) markers. A total of 85 10-mer primers were tested with the RAPD technique. A total of 76 polymorphic bands out of 272 bands (27.9%) were observed from 36 primers. The molecular size varied from 200 - 3000 bp. Genotypes were placed in nine different groups in the dendrogram drawn from the similarity matrix. The closest genotypes are ‘Yanako1’ and ‘Yanako2’. The relationship between the genotypes and their respective regions supported by the dendrogram is incongruent. Polymorphic information content (PIC) values ranged from 0.16 (OPD08) to 0.50 (OPC01 and OPC04) for the polymorphic data set. The larger source of variation was observed within populations (88.58%) followed by among populations (11.42%) in the analysis of molecular variance. The relationships among genotypes were defined by the first three principle components (PC), accounting for 40.91% of the total variation at the molecular level.

 

Key words: Ficus carica caprificus, genetic diversity, Aydın province, random amplified polymorphic DNA markers, fingerprinting, analysis of molecular variance,polymorphic information content, principle component analysis.

Abbreviation

Abbreviations: PCR-RFLP, Polymerase chain reaction-restriction fragment length polymorphism; RAPD, random amplified polymorphic DNA; AFLP, amplified fragment length polymorphism; CTAB, cethyl trimethyl ammonium bromide.

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