African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12486

Full Length Research Paper

Transferability of microsatellite loci from Croton floribundus Spreng. to Croton urucurana Baill. (Euphorbiaceae)

Fernanda Gliceria Alencar Motta
  • Fernanda Gliceria Alencar Motta
  • Biological Sciences, Dom Bosco Catholic University, Campo Grande, MS, Brazil.
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Leandro Skowronski
  • Leandro Skowronski
  • Postgraduate Program in Environmental Sciences and Agricultural Sustainability, Dom Bosco Catholic University, Campo Grande, MS, Brazil.
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Jannaína Velasques da Costa Pinto
  • Jannaína Velasques da Costa Pinto
  • Agro-forestry Sciences - Plant Genetics and Biotechnology, Federal University of Southern Bahia, Itabuna, BA, Brazil.
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Milene Silvestrini
  • Milene Silvestrini
  • Department of Plant Biology, Institute of Biology, UNICAMP, Campinas, SP, Brazil.
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Carina Elisei de Oliveira
  • Carina Elisei de Oliveira
  • Postgraduate Program in Environmental Sciences and Agricultural Sustainability, Dom Bosco Catholic University, Campo Grande, MS, Brazil.
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Paula Martin de Moraes
  • Paula Martin de Moraes
  • Postgraduate Program in Local Development, Dom Bosco Catholic University, Campo Grande, MS, Brazil.
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Reginaldo Brito da Costa
  • Reginaldo Brito da Costa
  • Postgraduate Program in Environmental Sciences and Agricultural Sustainability, Dom Bosco Catholic University, Campo Grande, MS, Brazil.
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  •  Received: 22 March 2019
  •  Accepted: 24 April 2019
  •  Published: 01 May 2019

Abstract

Croton urucurana, known as “sangra-d’água”, is a tree species with timber and beekeeping potential, and also with medicinal potential. The use of microsatellite markers, simple sequence repeats (SSRs) is efficient in population genetic studies, with high transferability rate between correlated species. The present study aimed to optimize the polymerase chain reaction (PCR) protocol and to test the transferability of seven primers developed for Croton floribundus to C. urucurana. The amplification of 113 accessions from different Brazilian biomes (Cerrado, Pantanal and Mata Atlântica) was tested. For optimization, the concentration and label of the MgCl2 reagent was varied in addition to annealing temperature. Four tested primers showed amplification products, guaranteeing 57% of transferability. This result confirms the efficiency of C. floribundus microsatellite loci for future studies of genetic variability in C. urucurana.
 
Key words: Genetic variability, sangra-d’água, molecular markers, simple sequence repeats (SSRs), primers.
 

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This article is published under the terms of the Creative Commons Attribution License 4.0

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