African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12227

Full Length Research Paper

Cytotoxic effect induced by Morinda morindoides leaf extracts in human and murine leukemia cells

OkiemyAkeli Marie-Genevieve1,5 , Ongoka Pascal Robin2, Gatouillat Gregory5, Attibayeba3, Lavaud Catherine4 and Madoulet Claudie*5
1Laboratoire de Biochimie et Biologie Moléculaire, Ecole Normale Supérieure, Université Marien NGOUABI, Brazzaville, B.P. 69, Congo. 2Unité de recherche de Chimie du Végétal et de la Vie (U2V), Faculté des Sciences, Université Marien NGOUABI, Brazzaville, B.P. 69, Congo. 3Unité de recherche de Physiologie Végétale, Faculté des Sciences, Université Marien NGOUABI,Brazzaville, B.P. 69, Congo. 4Laboratoire de Chimie Thérapeutique, UMR 6229, Institut de Chimie Moléculaire de REIMS ICMR, U.F.R. Pharmacie, IFR 53 Biomolécules, Université de Reims Champagne-Ardennes, Reims, France. 5Laboratoire de Biochimie et Biologie Moléculaire, U.F.R. Pharmacie, IFR 53 Biomolécules, Université de Reims Champagne-Ardennes, Reims, France.
Email: [email protected]

  •  Accepted: 25 June 2010
  •  Published: 27 September 2010

Abstract

We report here the in vitro effect of the toluene, methyl tert-buthyl ether (MtBE),ethyl acetate (EtOAc), n-buthanol (n-BuOH) and H2O  extracts from Morinda morindoides (rubiaceae) leaves against a human leukemic cell line, K562, and two murine leukemic cell lines, P388 and L1210. We found that both toluene and MtBE extracts exhibited a significant cytotoxic effect on these murine cell lines. The highest cytotoxicity was obtained with toluene extract in P388 (6.0 ± 1.4 µg/ml) and L1210 cells (6.5 ± 2.1 µg/ml). By contrast, EtOAc, n-BuOH and H2O extracts did not affect cell viability of the three cell lines tested. Effect of toluene and MtBE extracts was also examined on apoptosis induction in K562 human erythroleukemia cells. The results of this preliminary study have shown that the cytotoxic effects of toluene and MtBE extracts from Morinda morindoides leaves observed in K562 cells were mediated through the induction of apoptosis. Possible mechanisms responsible for the induction of apoptosis are discussed.

 

Key words: Morinda morindoides (Rubiaceae), cytotoxicity, apoptosis, leukemia cell lines, leaf extracts.

Abbreviation

MtBE: Methyl tert-butyl ether; EtOAc: ethyl acetate; n-BuOH: n-buthanol; MeOH: methanol; FBS: foetal bovine serum; DMSO: dimethyl sulfoxide;MTT, 3-(4,5-dimethylthiazolyl-2)-2,5-diphenyltetrazoliumbromide; PBS: phosphate-buffered saline; IC50: half maximal inhibitory concentration.