African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12487

Review

Molecular markers for genetic diversity and phylogeny research of Brazilian sheep breeds

Bruno do Amaral Crispim1*, Márcia Cristina Matos2, Leonardo de Oliveira Seno3and Alexéia Barufatti Grisolia1
  1Faculdade de Ciências Biológicas e Ambientais, Universidade Federal da Grande Dourados – Dourados/MS, Brazil. 2Faculdade de Ciências Agrárias e Veterinárias, Universidade Estadual Paulista – Jaboticabal/SP, Brazil. 3Faculdade de Ciências Agrárias, Universidade Federal da Grande Dourados – Dourados/MS, Brazil.
Email: [email protected]

  •  Accepted: 02 October 2012
  •  Published: 08 November 2012

Abstract

 

Brazilian sheep descended from several breeds brought to the New World by Portuguese and Spanish colonists, and they have evolved and adapted to local climatic variations and acquired tolerance or resistance to many diseases. Molecular markers are widely used in analyzing genetic variability, and markers such as amplified fragment length polymorphism (AFLP), restriction fragment length polymorphism (RFLP), microsatellite, mtDNA and single nucleotide polymorphism (SNP) have facilitated the characterization of genetic diversity and population structure, and in the investigation of the natural history, behavior, and evolution of several sheep breeds. In this context, we present here a review of the uses of molecular markers in ecological and conservation research of Brazilian sheep breeds.

 

Key words: DNA polymorphism, genetic conservation, Ovis aries

Abbreviation

SNP, single nucleotide polymorphism; PCR, polymerase chain reaction; AFLP, amplified fragment length polymorphism; PCR/RFLP, restriction fragment length polymorphism-polymerase chain reaction; RAPD, random amplified polymorphic DNA; STR, simple sequence repeats; SRY, sex-determining region Y;COI, cytochrome oxidase I