African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12182

Full Length Research Paper

Anti-Xanthomonas activity of Antarctic fungi crude extracts

Juliano Henrique Ferrarezi
  • Juliano Henrique Ferrarezi
  • Department of Biochemistry and Microbiology, Institute of Biosciences, São Paulo State University (UNESP) “Júlio de Mesquita Filho”, Av.24A, 1515, 13506-900, Rio Claro, SP-Brazil.
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Juliana Aparecida dos Santos
  • Juliana Aparecida dos Santos
  • Department of Biochemistry and Microbiology, Institute of Biosciences, São Paulo State University (UNESP) “Júlio de Mesquita Filho”, Av.24A, 1515, 13506-900, Rio Claro, SP-Brazil.
  • Google Scholar
Lara Durães Sette, Henrique Ferreira
  • Lara Durães Sette, Henrique Ferreira
  • Department of Biochemistry and Microbiology, Institute of Biosciences, São Paulo State University (UNESP) “Júlio de Mesquita Filho”, Av.24A, 1515, 13506-900, Rio Claro, SP-Brazil.
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Daiane Cristina Sass
  • Daiane Cristina Sass
  • Department of Biochemistry and Microbiology, Institute of Biosciences, São Paulo State University (UNESP) “Júlio de Mesquita Filho”, Av.24A, 1515, 13506-900, Rio Claro, SP-Brazil.
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  •  Received: 10 June 2019
  •  Accepted: 29 July 2019
  •  Published: 07 August 2019

Abstract

Agriculture suffers considerable losses in its production caused by plant diseases. In the citrus culture some problems were associated with citrus canker caused by Xanthomonas citri subsp. citri. That same way, sugarcane plantations are impaired to leaf scald disease caused by Xanthomonas albilineans. One of the measures used to contain citrus canker is the spray of cupric bactericides; however for scalding leaves there are no satisfactory control. Due to the phytosanitary problem caused by Xanthomonas (X. citri and X. albilineans) and by the difficulty of its control, the search for new forms of defense, less harmful to the environment, has become increasingly required. Thus, the aim of this work was to obtain crude organic extracts from filamentous fungi isolated from Antarctic soil samples and assess its bioactivity potential against X. citri and X. albilineans. One hundred and twenty-two extracts were tested, seven extracts inhibited the cell growth of X. citri, one was bioactive only against X. albilineans and one extract inhibited the cell growth of both bacteria. The bioactive extracts had a mean inhibition value of 96% against both bacteria. The values of MIC90 and MBC of bioactive extracts were also determined; for X. citri the isolate 3.1 Fe presented the lowest values of MIC90 (0.28 mg/mL) and MBC (1.0 mg/mL) and the two bioactive isolates for X. albilineans (1.1-Fe and B-Fe) presented the same values of MIC90 (1.4 mg/mL) and MBC (1.5 mg/mL). The filamentous fungi that produced positive extracts were identified as belonging to the genus Pseudogymnoascus (n=8) and Cladosporium (n=1). The filamentous fungi isolated from Antarctic soil produced compounds with bioactivity against phytopathogens from the Xanthomonas genus.

 

Key words: Antarctic fungi; antibacterial action; citrus canker; leaf scald; natural extracts.