African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12488

Full Length Research Paper

Production of single cell protein (SCP) and essentials amino acids from Candida utilis FMJ12 by solid state fermentation using mango waste supplemented with nitrogen sources

Marius Kounbesioune SOMDA
  • Marius Kounbesioune SOMDA
  • Laboratory of Microbiology and Microbial Biotechnology, Research Center in Biological Food and Nutrition Sciences (CRSBAN), Department of Biochemistry and Microbiology, University Ouaga1 Pr Joseph KI-ZERBO, Burkina Faso.
  • Search for this author on:
  • Google Scholar
Mahamadi NIKIEMA
  • Mahamadi NIKIEMA
  • Laboratory of Microbiology and Microbial Biotechnology, Research Center in Biological Food and Nutrition Sciences (CRSBAN), Department of Biochemistry and Microbiology, University Ouaga1 Pr Joseph KI-ZERBO, Burkina Faso.
  • Search for this author on:
  • Google Scholar
Ibrahim KEITA
  • Ibrahim KEITA
  • Laboratory of Applied Molecular Biology (LBMA), Faculty of Technics and Sciences, University of Sciences, Technics and Technologies of Bamako, BP: E 3206, Colline de Badalabougou, Bamako, Mali.
  • Search for this author on:
  • Google Scholar
Iliassou MOGMENGA
  • Iliassou MOGMENGA
  • Laboratory of Microbiology and Microbial Biotechnology, Research Center in Biological Food and Nutrition Sciences (CRSBAN), Department of Biochemistry and Microbiology, University Ouaga1 Pr Joseph KI-ZERBO, Burkina Faso.
  • Search for this author on:
  • Google Scholar
Sonagnon H. S. KOUHOUNDE
  • Sonagnon H. S. KOUHOUNDE
  • Laboratory of Microbiology and Food Technology, Faculty of Sciences and Technics, University of Abomey-Calavi, 04 BP 1107 Cotonou, Benin.
  • Search for this author on:
  • Google Scholar
Yerobessor DABIRE
  • Yerobessor DABIRE
  • Laboratory of Microbiology and Microbial Biotechnology, Research Center in Biological Food and Nutrition Sciences (CRSBAN), Department of Biochemistry and Microbiology, University Ouaga1 Pr Joseph KI-ZERBO, Burkina Faso.
  • Search for this author on:
  • Google Scholar
Wahauwouele H. COULIBALY
  • Wahauwouele H. COULIBALY
  • Laboratory of Biotechnology and Food Microbiology, UFR-STA, University of Nangui Abrogoua, Ivory Coast.
  • Search for this author on:
  • Google Scholar
Essodolom TAALE
  • Essodolom TAALE
  • Laboratory of Microbiology and Food Quality, High School of Biological and Food technics (ESTBA), University of Lome BP: 1515, Togo.
  • Search for this author on:
  • Google Scholar
Alfred S.TRAORE
  • Alfred S.TRAORE
  • Laboratory of Food Technology, Department of Biochemistry and Microbiology, University Ouaga1 Pr Joseph KI-ZERBO, Burkina Faso.
  • Search for this author on:
  • Google Scholar


  •  Received: 10 December 2017
  •  Accepted: 27 March 2018
  •  Published: 06 June 2018

Abstract

In Burkina Faso, deficiency of amino acids in protein is becoming a major healthy public problem. This study was purposed to optimize essentials amino acids in single cell protein (SCP) by supplementing different nitrogen sources during fermentation of mango waste with Candida utilis FJM12. Analytical methods were used to determine biomass yield, chemical composition and amino acids profile of SCP. The principal component analysis (PCA) method was performed to identify the nitrogen source which exhibited best rate of SCP. The maximum biomass yield (6.48±0.03 g/L) exhibited 9.65±0.36% (w/w) of ash, while using yeast extract. The proximate composition of SCP revealed 56.40±1.30, 13.25±0.11, 3.80±0.10, and 6.60±0.25% (w/w), respectively for crude protein, lipids, carbohydrates, and nucleic acid content. PCA showed a strong correlation between yeast extract and Ammonium sulphate and demonstrated their positive influence to increase the rate of SCP and essentials amino acids as compared to Food and Agriculture Organization (FAO) recommendation. These results demonstrated that C. utilis FJM12 could be suitable for essentials amino acids.

Key words:  Mango waste, nitrogen source, Candida utilis, single cell protein (SCP), amino acids.

 

Copyright © 2024 Author(s) retain the copyright of this article.

This article is published under the terms of the Creative Commons Attribution License 4.0

Back to Vol. 17 No. 23
Back to articles
SUBSCRIBE TO RSS
SUBMIT MANUSCRIPT
CONFERENCES