African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12487

Full Length Research Paper

Selection of ligand peptides with the ability to detect antibodies in enzootic bovine leukosis

  Elizangela Maira dos SANTOS1*,  Rone CARDOSO2, Luiz Ricardo GOULART FILHO2, Marcos Bryan HEINEMANN1, Rômulo Cerqueira LEITE1 and Jenner Karlisson Pimenta dos REIS1      
  1Departamento de Medicina Veterinária Preventiva, Escola de Veterinária – Universidade Federal de Minas Gerais (UFMG) 30 123-970, Belo Horizonte, MG, Brasil. 2Instituto de Genética e Bioquímica, Laboratório de Nanobiotecnologia, Campus Umuarama – Universidade Federal de Uberlândia (UFU) 38 400-902, Uberlândia, MG, Brasil.
Email: [email protected]

  •  Accepted: 22 March 2012
  •  Published: 05 April 2012

Abstract

 

Peptides present in phages were selected using phage display technology and immunoassays to find out the antigenic mimetics of immunodominant epitopes of bovine leukosis virus (BLV). The use of antigenic mimetics may result in the enhancement of the sensitivity and specificity of the serologic diagnosis of enzootic bovine leukosis (EBL), contributing directly to disease control. The selections enabled the choice of clones which can be used as potential antigens in the diagnoses of diseases. The synthetic peptide produced from the selected sequences may be considered as an alternative for antigens in the serologic diagnosis of enzootic bovine leukosis.

 

Key words: Diagnostic, antigens, enzootic bovine leukosis (EBL), phage display.

Abbreviation

Abbreviations: AGID, agar gel immunodiffusion; BLV, Bovine leukosis virus; EBL,enzootic bovine leucosis; ELISA, enzyme-linked immunosorbent assay; PCR,polymerase chain reaction.

Copyright © 2024 Author(s) retain the copyright of this article.

This article is published under the terms of the Creative Commons Attribution License 4.0

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