African Journal of

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12484

Full Length Research Paper

Response of Glycine max in relation to nitrogen fixation as influenced by fungicide seed treatment

Anupama Bikrol, Nidhi Saxena and Kiran Singh*
Laboratory of Bioenergetics, Department of Microbiology, Barkatullah University, Bhopal-462026, India
Email: [email protected]

  •  Accepted: 08 March 2005
  •  Published: 31 July 2005



Glycine max – Rhizobium interaction is a well known symbiotic association occurring in nature and responsible for biological nitrogen fixation. Thiram a well-known fungicide has been in practice as seed dressing in order to prevent fungal colonization. In the present study the effect of various thiram concentrations is investigated. Thiram concentration beyond 500 μg/ml was observed to be highly toxic with respect to plant growth factors and rhizobial infection to the G. max. The nodulation, nodule dry weight, nitrogenase activity were observed to be maximum at 100 μg/ml of thiram. The study is useful in determining the threshold concentration of fungicide for soybean seed dressing for effective nitrogen fixation and crop yield.


Key words: Ascorbic acid, chlorophyll content, Glycine max, nodulation, nodule dry weight, nitrogenase activity, protein content, thiram.