African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12258

Full Length Research Paper

In vitro regeneration of Calophyllum brasiliense Cambess: A valuable medicinal tree

A. Maldonado-Magana
  • A. Maldonado-Magana
  • Centro de Investigaciones Quimicas, Universidad Autonoma del Estado de Morelos. Av. Universidad 1001, Col. Chamilpa C.P. 62209, Cuernavaca, Morelos, Mexico.
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A. Bernabe-Antonio*
  • A. Bernabe-Antonio*
  • Departamento de Madera, Celulosa y Papel. Centro Universitario de Ciencias Exactas e Ingenierias, Universidad de Guadalajara, Km. 15.5. Carretera Guadalajara-Nogales, Las Agujas, C.P. 45020, Zapopan, Jalisco, Mexico. Tel: +52 3336 820110, ext. 202.
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E. Salcedo-Perez
  • E. Salcedo-Perez
  • Departamento de Madera, Celulosa y Papel. Centro Universitario de Ciencias Exactas e Ingenierias, Universidad de Guadalajara, Km. 15.5. Carretera Guadalajara-Nogales, Las Agujas, C.P. 45020, Zapopan, Jalisco, Mexico. Tel: +52 3336 820110, ext. 202.
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F. Cruz-Sosa
  • F. Cruz-Sosa
  • 3Departamento de Biotecnologia, Universidad Autonoma Metropolitana-Iztapalapa, San Rafael Atlixco 186, CP. 09340 México D.F, Mexico.
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  •  Received: 14 July 2015
  •  Accepted: 30 September 2015
  •  Published: 07 October 2015

Abstract

Calophyllum brasiliense (Calophyllaceae) is a medicinal tree known mainly for producing calanolides, secondary metabolites against HIV-1 reverse transcriptase. This wild plant is listed as threatened and despite its outstanding medicinal value, no studies have been conducted on its propagation or preservation. This study standardized a procedure for the micropropagation of C. brasiliense with nodal segments from in vitro seedlings. The in vitro seed germination was 48.6%. The nodal explants displayed a high percentage of shoot induction (77.5%), shoots per segment (6.9), nodes per shoot (3.8), leaves per shoot (8.0) and shoot length (4.2 cm) when 0.5 mg L-1 indole-3-butyric acid plus 0.1 mg L-1 thidiazuron were used. Furthermore, maximum shoot rooting (63.5%) and root length (2.2 cm) were recovered using 1.0 mg L-1 indole-3-acetic acid. More than three-quarters of the acclimatized plants (77.5%) grew successfully in pots. Thus, this study developed an in vitro propagation protocol for C. brasiliense that can be used as a potential resource for restoring wild populations or performing phytopharmacological studies.
 
Key words: Seed germination, plant growth regulators, micropropagation, nodal segments, medicinal plants.
 

Abbreviation

BA, 6-Benzyladenine; IBA, indole-3-butyric acid; TDZ, thidiazuron; MS, Murashige and Skoog; PGRs, plant growth regulators; IAA, indole-3-acetic acid.