African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12486

Effects of benzylaminopurine and naphthalene acetic acid on proliferation and shoot growth of pineapple (Ananas comosus L. Merr) in vitro

Adel M. Al-Saif*1, A. B.M. Sharif Hossain2 and Rosna Mat Taha2        
1Department of Plant Production, College of Food & Agricultural Sciences, King Saud University, P.O.Box  2460,  Riyadh 11451 , Saudi Arabia. 2Biotechnology Division, Institute of Biological Sciences,Faculty of Science, University of Malaya, 50603 KualaLumpur, Malaysia.  
Email: [email protected]

  •  Accepted: 25 April 2011
  •  Published: 15 June 2011

Abstract

This study was conducted to evaluate the pineapple regeneration and shoot growth as affected by 6-benzylaminopurine (BAP) at 2.0 mg/l and naphthalene acetic acid (NAA) at 0.2 mg/l in vitro. BAP and NAA at the concentration of 2.0 and 0.2 mg/l were used in this study. BAP at 2.0 mg/l significantly affected the production of shoots per explant, shoot length and weight. Total shoot length was higher in BAP (2 mg/l) than in control (MS medium without hormone) and NAA (0.2mg/l) after 10, 20, 30, 40, 50 and 60 days incubation period. Total shoot length was highest in BAP in all incubation periods. Total shoot weight was higher in BAP (2 mg/l) and lower in NAA (0.2 mg/l) as compared to MS medium without hormone. The results showed that BAP at the concentration of 2 mg/l was effective for pineapple shoot growth and development.

 

Key words: Pineapple regeneration, shoot growth, 6-benzylaminopurine, naphthalene acetic acid.