June 2011
Quantification of virus syndrome in chili peppers
One of the most important problems to produce chili crops is the presence of diseases caused by pathogen agents, such as viruses, therefore, there is a substantial necessity to better predict the behavior of the diseases of these crops, determining a more precise quantification of the disease’s syndrome that allows the investigators to evaluate better practices, from handling to the experimental level...
June 2011
Genetic Variation in Captive Herd of Arabian Oryx Using RAPD and ISSR Markers
According to the international taxonomy of IUCN, the Arabian Oryx (Oryx leucoryx) is considered to be in endangered and included among the animals that may be extinct. Saudi Arabia began an important project of captive breeding of this species in 1986 at the National Wildlife Research Center (NWRC). Therefore, the current captive population of Saudi Arabian Oryx at NWRC may suffer from inbreeding depression...
June 2011
A genetic polymorphism and its genetic effects on goat myogenin gene in intron 1
Single nucleotide polymorphisms (SNPs) of the myogenin (MyoG) gene were tested using primer induced restriction fragment length polymorphism assay-polymerase chain reaction (PIRA-PCR) from Bore goat and its upgrading offspring to Tangshan diary goat (including F1, F2 and F3). The effects of the myogenin gene on the birth weight, 1-month body weight and the weaning weight were also analyzed....
June 2011
Assessment of genetic diversity among collected genotypes of Chlorophytum borivilianum using random amplified polymorphic DNA (RAPD) markers
Chlorophytum borivilianum (Family: Liliaceae), an important threatened medicinal herb is designated as ‘Rare’ in Red Data Book of Indian plants. Morphological as well as biochemical markers used in the authentication of herbal drugs have many limitations due to the impact of environmental conditions. Molecular markers therefore, are an important tool in quality assurance and preservation of...
June 2011
A novel CYP1A1 gene polymorphism and the risk of head and neck cancer in Pakistani population
Several polymorphisms in the CYP1A1 locus have been identified and their genotypes appear to exhibit population frequencies that depend on ethnicity. In this study, we assessed the role of CYP1A1 genotype in 388 head and neck cancer patients in Pakistani population via a case-control study. Polymerase chain reaction (PCR) and single stranded conformational polymorphism...
June 2011
Local cabbage (Brassica oleracea var. capitata L.) populations from Serbian Province of Vojvodina
In previous experiments, we were able to augment cabbages (Brassica oleracea L. var. capitata L.) with two new local open pollinated (OP) populations and one cultivar.The type of use indicated that these are cabbages with thinner and juicier leaves, which predisposes their heads for fine grating and also makes their leaves readily bendable and easy to roll up when...
June 2011
Consistent association of fungus Fusarium mangiferae Britz with mango malformation disease in Pakistan
Mango malformation disease (MMD) deforms the natural shape of panicles and shoots. The disease incitant is of great concern due to its complexity and mode of infection. Recently, a new species Fusarium mangiferae Britz was confirmed as the etiological agent of MMD in African and Asian clade. There was a need to confirm the fungus in other Asian countries. We investigated the association...
June 2011
Effects of benzylaminopurine and naphthalene acetic acid on proliferation and shoot growth of pineapple (Ananas comosus L. Merr) in vitro
This study was conducted to evaluate the pineapple regeneration and shoot growth as affected by 6-benzylaminopurine (BAP) at 2.0 mg/l and naphthalene acetic acid (NAA) at 0.2 mg/l in vitro. BAP and NAA at the concentration of 2.0 and 0.2 mg/l were used in this study. BAP at 2.0 mg/l significantly affected the production of shoots per explant, shoot length and weight. Total shoot length was higher in BAP (2...
June 2011
Construction and analysis of a suppression subtractive hybridization (SSH) library of genic multiple-allele inherited male-sterility in Chinese cabbage (Brassica campestris L. ssp. pekinensis)
Utilization of male sterility is a key method for producing crossbred Chinese cabbage (Brassica rapa L. ssp. pekinensis (Lour.) Olsson. In this study, suppression subtractive hybridization (SSH) was used to construct sterility and fertility cDNA libraries, which included differentially, expressed clones between fertile and sterile buds of the A/B line ‘AB01’. The positive clones were...
June 2011
Some chemical and physical properties at physiological maturity and ripening period of kiwifruit (‘Hayward’)
This study was carried out to determine some chemical and physical properties atphysiological maturity and ripening period of kiwifruit. The average geometric mean diameter, sphericity, bulk density, porosity, projected area along three axes (X, Y, Z) and colour characteristics (L*, a*, b*) were measured at physiological maturity and ripening period. Total soluble solid...
June 2011
The effects of boron management on soil microbial population and enzyme activities
Boron is an essential micronutrient required for plant growth. Soil microorganisms directly influence boron content of soil as maximum boron release corresponds with the highest microbial activity. The objective of this study is to determine the effects of different levels of boron fertilizer on microbial population, microbial respiration and soil enzyme activities in different soil depths in cultivated wheat...
June 2011
Biohydrogen production from diary processing wastewater by anaerobic biofilm reactors
Fermentative hydrogen production was studied in packed bed batch reactors to assess the influence of environmental factors over yield hydrogen production from dairy wastewater. Dried stems of Opuntia imbricata were used as substratum adding a pretreated mixed culture for biofilm formation. Experimental results showed that, yield hydrogen production was significantly affected by initial...
June 2011
Isolation and drought-tolerant function analysis of ZmPti1-1, a homologue to Pti1, from maize (Zea mays L.)
Pto-interacting 1(Pti1) has been well established to play important roles in plant disease and salt response, but its potential roles in the response to drought stress is unknown. In this study, the Pti1-like gene named as ZmPti1-1 was cloned from maize, sequence analysis showed that ZmPti1-1 encodes a polypeptide of 363 amino acids with predicted molecular mass of 39.0 kDa and an isoelectric...
June 2011
L-Glutamic acid production by Bacillus spp. isolated from vegetable proteins
22 isolates of Bacillus species were obtained from “Dawadawa”, “Ugba” and “Ogiri” (fermented vegetable proteins) in Nigeria. The isolates were identified as Bacillus subtilis (6), (27.3%), Bacillus pumilus (5), (22.7%), Bacillus licheniformis (5), (27.3%) and Bacillus polymyxa (6), (22.7%). Four species of...
June 2011
Effect of various carbon and nitrogen sources on cellulose synthesis by Acetobacter lovaniensis HBB5
The effect of various carbon and nitrogen sources on cellulose production byAcetobacter lovaniensis HBB5 was examined. In this study, glucose, fructose, sucrose and ethanol as carbon source and yeast extract, casein hydrolysate and ammonium sulphate as nitrogen source were used. Among the carbon sources, glucose gave the highest yield, followed by fructose, sucrose and ethanol. Besides, among the nitrogen...
June 2011
The effects of calcium regulation of endosperm reserve protein mobilization of the Nigeria sorghum cultivars, ICSV 400 and KSV 8 during malting
The effects of steep liquor calcium ion on sorghum endosperm reserve protein mobilization were evaluated using two improved Nigeria sorghum cultivars (ICSV 400 and KSV 8). The key protein modification factors evaluated were free amino nitrogen(FAN), total non protein nitrogen (TNPN) and soluble protein of cold water extract (CWS-P). Ca2+ treatment highly significantly (P < 0.001) repressed FAN...
June 2011
Norcantharidin (NCTD) induces mitochondria mediated apoptosis in human HepG2 cells
Norcantharidin (NCTD), a demethylated form of cantharidin, is now in used as a routine anticancer drug. However, the detailed mechanisms underlying this process are generally unclear. The aims of this study were to evaluate the apoptotic effects and molecular mechanisms of NCTD. MTT assay was used to determine the cell growth inhibitory rate. Flow cytometry were used to detect the apoptosis and the loss of mitochondrial...
June 2011
Phytotoxic characterization of various fractions of Launaea procumbens
Allelopathic screening of various fractions of Launaea procumbens, collected from Wah Cantt (Punjab) Pakistan, was conceded to identify potent allelopathic fraction for future phytochemical analyses. For this purpose, radish root inhibition method was used to test allelopathic potential. Two different concentrations of 100 ppm and 1000 ppm of Launaea procumbens were used in this study. Methanolic and...
June 2011
Kenaf seed oil from supercritical carbon dioxide fluid extraction shows cytotoxic effects towards various cancer cell lines
Hibiscus cannabinus (Kenaf) from the family of Malvaceae is a valuable fiber source and medicinal plant. It has long been prescribed as traditional folk medicine in Africa and India to treat various diseases. Nevertheless, little research has been carried out on the potentials of this plant as treatment for cancer. This study was designed to determine the cytotoxicity of kenaf seed oil from two...
June 2011
Kenaf seed oil from supercritical carbon dioxide fluid extraction induced G1 phase cell cycle arrest and apoptosis in leukemia cells
This study was designed to determine the cytotoxic effects of kenaf seed oil (Hibiscus cannabinus) variety V36 extracted using supercritical carbon dioxide fluid extraction (SFE) with different combinations of pressure (bars) and temperature (°C). Extracted oils were tested on human promyelocytic HL-60, murine myelomonocyticWEHI-3B and human chronic myelogenous K562 leukemic cell...
June 2011
Study of Salmonella typhimurium mutagenicity assay of (E)-piplartine by the Ames test
Phytochemical studies carried out with Piperaceae species have shown great diversity of secondary metabolites among which are several displayed considerable biological activities. The species Piper tuberculatum has been intensively investigated and a series of amides have been described. For instance, (E)-piplartine showed significant cytotoxic activity against tumor cell lines, especially human leukemia cell...
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