African Journal of

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12269

Full Length Research Paper

Characterization of a hemorrhage-inducing component present in Bitis arietans venom

Tahis Louvain de Souza
  • Tahis Louvain de Souza
  • Laboratorio de Biologia do Reconhecer, Centro de Biociencias e Biotecnologia, Universidade Estadual do Norte Fluminense Darcy ribeiro, Campos dos Goytacazes, RJ, Brazil.
  • Google Scholar
Fabio C. Magnoli
  • Fabio C. Magnoli
  • Laboratorio de Imunoquimica, Instituto Butantan, Sao Paulo, SP , Brazil.
  • Google Scholar
Wilmar Dias da Silva*
  • Wilmar Dias da Silva*
  • Laboratorio de Imunoquimica, Instituto Butantan, Sao Paulo, SP , Brazil.
  • Google Scholar

  •  Received: 13 November 2014
  •  Accepted: 16 February 2015
  •  Published: 25 March 2015


Better characterization of individual snake venom toxins is useful for analyzing the association of their toxic domains and relevant antigenic epitopes. Here we analyzed the Bitis arietans hemorrhagic-inducing toxin present in a representative venom sample. Among the 1´ to 5´ protein peaks isolated using a Sephacryl S 100 HR chromatography column, hemorrhagic activity was expressed by all according to the following intensity, P´5 > P´3 > P´2 >, P´4. The proteins were recognized and measured with antibodies present in polyvalent horse F(ab)´2 anti-B. arietans, Bitis spp., Lachesis muta, B. atrox, Bothrops spp., Crotalus spp., and Naja spp. or in IgY anti-B. arietans and anti-Bitis spp. using enzyme-linked immunosorbent assays and western blotting. In addition, in an in vitro-in vivo assay these anti-venoms were able to block hemorrhagic-inducing activity. The evident cross-reactivity expressed by different specific anti-venoms indicates that metalloproteinases induce an immunological signature indicating the presence of similar antigenic epitopes for several snake venoms.
Key words: Snake venoms, anti-venoms, Bitis arietans, hemorrhage, metalloproteinases.