African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12487

Full Length Research Paper

Molecular characterization of Fusarium oxysporum f. sp. ciceri causing wilt of chickpea

Bhim Pratap Singh1*, Ratul Saikia2, Mukesh Yadav1, Rakesh Singh1, V.S.Chauhan3 and Dilip K. Arora1
1National Bureau of Agriculturally Important Microorganisms, Kushmaur, Mau 275101, India. 2Division of Biotechnology, Regional Research Laboratory (CSIR), Jorhat 785006, India 3Department of Biotechnology, Bundelkhand University, Jhansi, India.
Email: [email protected]

  •  Accepted: 16 February 2006
  •  Published: 15 March 2006

Abstract

Thirty isolates of Fusarium oxysporum f. sp. ciceri were isolated from rhizosphere soil of chickpea from different locations in Northern India. The amount of genetic variation was evaluated by polymerase chain reaction (PCR) amplification with a set of 40 RAPD primers and 2 IGS primers. Less than 10% of the amplified fragments in each case were polymorphic. Genetic similarity between each of the isolates was calculated and results indicate that there was little genetic variability among the isolates collected from the different locations. At the 0.75 similarity index the isolates divides into three groups. Isolates Foc-A18, Foc-A19,Foc-A20 forming a similar group and far different from other isolates.

 

Key words: Fusarium oxysporum f. sp. ciceri, Fusarium wilt of chickpea, RAPD, ITS, IGS.

Copyright © 2024 Author(s) retain the copyright of this article.

This article is published under the terms of the Creative Commons Attribution License 4.0

Back to Vol. 5 No. 6
Back to articles
  • Articles on Google by:
SUBSCRIBE TO RSS
SUBMIT MANUSCRIPT
CONFERENCES