March 2006
Cloning and characterization of maize ZmSPK1, a homologue to nonfermenting1-related protein kinase2
SnRK2s play important roles in plant stresses responses. One full-length cDNA encoding a SnRK2b homologue was isolated from maize by RT-PCR and named asZmSPK1 (for stress-induced protein kinase). The ZmSPK1 protein has 364 amino acids with an estimated molecular mass of 41.8 KD and an isoelectric point of 5.8. The deduced protein sequence has the closest identities to the members of SnRK2b...
March 2006
Molecular characterization of Fusarium oxysporum f. sp. ciceri causing wilt of chickpea
Thirty isolates of Fusarium oxysporum f. sp. ciceri were isolated from rhizosphere soil of chickpea from different locations in Northern India. The amount of genetic variation was evaluated by polymerase chain reaction (PCR) amplification with a set of 40 RAPD primers and 2 IGS primers. Less than 10% of the amplified fragments in each case were polymorphic. Genetic similarity between each of the...
March 2006
Conversion of masonia tree sawdust and cotton plant by product into feed by white rot fungus (Pleurotus sajor caju)
The influence of fungus treatment on the biochemical composition and degradation patter of sawdust and cotton plant by-products (cotton burns and cotton gin trash) byPleurotus sajor caju were evaluated. Lignin degradation increased as the incubation period progressed while the highest loss of hemicellulose, cellulose, acid detergent fibre, neutral detergent fibre were recorded for treated cotton plant...
March 2006
Growth and lactic acid production by Bifidobacterium longum and Lactobacillus acidophilus in goat’s milk
The fitness of a particular strains of Bifidobacteria, Lactobacilli and Streptococci for commercial utilization depends on its rapid growth and acidification of milk as well as its acid and oxygen tolerence. From 20 samples of French commercial yoghurt, one species of bifidobacteria was identified as Bifidobacterium longum. Also,Lactobacillus acidophilus was isolated and identified from...
March 2006
Arbuscular mycorrhizal fungi species associated with rhizosphere of Phoenix dactylifera L. in Morocco
A survey of arbuscular mycorrhizal fungi (AMF) diversity and date palm (Phoenix dactylifera L.) tree root colonization in arid areas was undertaken in ten palm groves located along the Ziz valley (Tafilalet, south-west Morocco). The frequency and the mean intensity of root colonization reached 72 and 43% respectively and the spore population varied from 238 to 1840 spores/10g of soil. The AMF colonization...
March 2006
Feasibility of cheese production and whey valorization in the Adamawa Province of Cameroon
Problems associated with perishability and distribution of local milk and milk products by small-scale producers in the Adamawa province of Cameroon, justified development of an easy process for producing pressed-dough cheese and flavoured whey. Production from raw milk was technically profitable with mean cheese and flavoured whey yields of 8.9 and 85,6% (w/w), respectively. The cheese and whey comprise 50 and 45.7 %...
March 2006
Patterns of resistance to β-lactams and β-lactamase inhibitors in uropathogenic Escherichia coli strains isolated from animals in Portugal
FMV 1953 uropathogenic Escherichia coli isolate was extremely resistant to amoxicillin, co-amoxiclav, ticarcillin, mecillinam, cefoxitime, cefixime, cefuroxime, cefotaxime, ceftazidime, ceftriaxone and aztreoname. From the resistance patterns observed we deduce the phenotype as producing a TEM-1 β -lactamase, a hyperproduced Amp-C β-lactamase and an OXA-3 β-lactamase or a PBP-2 mutation...
March 2006
Further screening of Venda medicinal plants for activity against HIV type 1 reverse transcriptase and integrase
The use of medicinal plants for AIDS-related conditions is common in South Africa. In order to establish an antiviral rationale for the use of these plants we screened fractions of the methanol extracts of medicinal plants for activity against HIV-1 reverse transcriptase (RT) and integrase (IN). The n-butanol fraction obtained from the crude methanol extracts of the roots of Bridelia micrantha (Hochst) Baill....
March 2006
Phytochemical screening and anti-inflammatory evaluation of methanolic and aqueous extracts of Euphorbia heterophylla Linn (Euphorbiaceae)
Euphobia heterophylla is a local medicinal plant used in ethnomedicine for the treatment of constipation, bronchitis and asthma. The aqueous decoction and the methanolic extracts were subjected to anti-inflammatory activity using experimental animal model, in the presence of the positive control drugs. The inflammation was induced by carraegenean. From the results obtained the aqueous extract showed significant...
March 2006
Pilot study of DNA extraction from archival unstained bone marrow slides: comparison of three rapid methods
DNA can be isolated from a variety of human sample sources including anti-coagulant whole blood, bloodstains, hairs, tissue samples and buccal epithelial cells. The purpose of this study was to compare yield and quality of DNA samples obtained with the use of three different methods. The ability of these procedures to provide DNA for polymerase chain reaction (PCR) amplification from archival unstained bone marrow...
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