African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12227

Full Length Research Paper

Enhanced regeneration in explants of tomato (Lycopersicon esculentum L.) with the treatment of coconut water

Amber Afroz1, Zubeda Chaudhry2, Umer Rashid3, Muhammad Rashid Khan1and Ghulam Muhammad Ali3
  1Department of Biochemistry, Quaid-i-Azam University, Islamabad. 2Department of Botany, Hazara University, Mansehra, Pakistan. 3Plant Biotechnology Program National Agriculture Research Center Park Road, Islamabad, Pakistan.
Email: [email protected]

  •  Accepted: 14 May 2010
  •  Published: 14 June 2010

Abstract

 

A standardized protocol was developed to enhance the in vitro regeneration efficiency of five varieties of tomato from hypocotyls and leaf disc with the involvement of coconut water. Different concentrations of IAA and kinetin were used alone and in combination with 12% of coconut water. Significant differences for regeneration, time taken to regenerate and number of leaf primordial were observed for different treatments, type of explant, use of coconut water and also among varieties. Higher regeneration was obtained in Avinash followed by Roma and Rio Grande. Maximum regeneration 95.75% was obtained with 0.5 mgL-1 of IAA, 1.5 mgL-1 Kinetin along with 12% coconut water in Avinash and hypocotyl was proved to be better for regeneration than leaf discs. The inclusion of coconut water in the media significantly reduced the number of days taken for callus induction leading to regeneration, as compared to media without coconut water. Significant increase in number of shoot primordial was observed in hypocotyls as well as in leaf disc derived calli with the addition of coconut water. Plants obtained were transferred to the glass house in small pots of compost and higher frequency of survival was observed from plantlets obtained with the addition of coconut water.

 

Key words: Tomato, coconut water, regeneration, days to maturity, shoot primordia.

Abbreviation

CW, Coconut water; IAA, indole acetic acid; BAP6-benzylaminopurine.