African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12487

Full Length Research Paper

Assessment of genetic and biochemical diversity of ecologically variant ectomycorrhizal Russula sp. from India

M. K. Upadhyay1*, Devendra Jain1, Abhijeet Singh1, A. K. Pandey2 and R. C. Rajak1
1School of Life Sciences, Jaipur National University, Jagatpura, Jaipur, India-302025. 2Department of Biosciences, Rani Durgavati University, Jabalpur, India.
Email: [email protected]

  •  Accepted: 01 March 2010
  •  Published: 22 March 2010

Abstract

The aim of this study was to develop the phylogenetic relationship amongst the ecologically variant Russula species by using polymerase chain reaction (PCR) based technique, random amplified polymorphic DNA (RAPD) and isozyme analyses. Two groups could be characterized amongst the total isolates by cluster analyses. Protease, cellulase, glutamate dehydrogenase, pectinase and acid phosphatase designated band P220.16, C472.18, GLD130.21, Pe569.12 and AP472.12, respectively, were common in all the isolates and four monomorphic RAPD bands viz; 818, 512, 298 and 201 bp were also diversified in the isolates. This common band reveals that diversity of these alleles or loci in all ecologically variant isolates. Thus, the present studies discuss the genetic diversity of ecologically variantRussula species on the basis of RAPD and isozyme analysis.

 

Key words: Genetic diversity, isozyme, random amplified polymorphic DNA,Russula sp., zymography.

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This article is published under the terms of the Creative Commons Attribution License 4.0

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