African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12182

Full Length Research Paper

Body extract of tail amputated zebrafish promotes culturing of primary fin cells from glass catfish

Jee Eun Han1, Ji Hyung Kim1,2, Casiano Choresca1, Sang Phil Shin1, Jin Woo Jun1and Se Chang Park1*
  1Laboratory of Aquatic Biomedicine, College of Veterinary Medicine and Research Institute for Veterinary Science, Seoul National University, Seoul 151-742, Republic of Korea. 2Korea Ocean Research and Development Institute, Ansan, 426-744, Republic of Korea.
Email: [email protected]

  •  Accepted: 17 December 2012
  •  Published: 31 March 2013

Abstract

 

The most spectacular regenerative events in vertebrates are epimorphic regeneration. In this study, interestingly, a whole-body extract 24 h after tail amputation enhanced primary cell growth and viability compared to that of a non-tail amputated body. Additionally, these effects of extract treatment in vitro were dose-dependent occurring at concentrations of 0.02, 0.05 and 0.1 mg/ml. This is the first in vitro study on the interaction between primary fin cells from glass catfish and tail amputated body extracts of zebrafish. These results provide an essential knowledge base for rational approaches to tissue and organ regeneration.

 

Key words: Cell growth, cell viability, extract, glass catfish, regeneration, zebrafish.

Abbreviation

DMEM,  Dulbecco’s  modified  eagle’s  medium; P/S, penicillin-streptomycin.

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