African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12083

Table of Content: 20 March 2013; 12(12)

March 2013

Natural rubber producing plants: An overview

  Currently, Hevea brasiliensis has been only one resource for commercial natural rubber production. Rubber tree, H. brasiliensis (Willd. ex A. Juss.) Muell. Arg., commonly known as the Brazilian rubber tree is native to the Amazon River basin.Attempts to develop alternative sources of natural rubber have been made at various times and no fewer than eight botanical families, 300 genera...

Author(s): Perumal Venkatachalam, Natesan Geetha, Palanivel Sangeetha and Arjunan Thulaseedharan

March 2013

Protective effect and expression of defense-related ESTs induced by acibenzolar-S-methyl and a phosphorylated mannan oligosaccharide-based product against Moniliophthora perniciosa in Theobroma cacao

  Witches’ broom disease (WBD), caused by the fungus Moniliophthora perniciosa, is one of the main diseases in cocoa (Theobroma cacao) and has caused severe economic losses. Integrated disease management has been the focus for its control and therefore, the identification of new inducers of plant resistance is desirable. Thus, the goal of this work was to evaluate two potential inducers of...

Author(s): Lívia de Matos Pereira, Mário Lúcio Vilela de Resende, Sandra Marisa Mathioni, Magnólia Araújo Campos, Pedro Martins Ribeiro Júnior, Fabrício Rabelo Camilo, Anderson Tadeu Silva and Luciano Vilela Paiva

March 2013

Common mutations in G6PD of Vietnamese-Kinh deficient patients

  This study was conducted to identify the common mutations occurring within theG6PD gene in the G6PD - Vietnamese deficient patients, which may be the main causative mutations of the G6PD deficiency in Vietnamese-kinh. Sequencing was performed to detect mutations in the coding region of G6PD gene for 30 Vietnamese-Kinh G6PD deficient patients. Seven sets of primers were designed to amplify and...

Author(s): Nguyen Thi Hue, Dang ThiLan Anh, Huynh Le Thao Trinh and Phan Ngo Hoang

March 2013

Characterization of Quercus species distributed in Jordan using morphological and molecular markers

  Genetic diversity among 25 natural populations of three different species of Quercusin Jordan at morphological and molecular levels using random amplified polymorphic DNA (RAPD) primers was assessed. Significant morphological and molecular variations among and within 25 Quercus populations were estimated. Standardized canonical discrimination functions for the investigated morphological traits...

Author(s): Mohammad S. Jawarneh, Mohammad H. Brake, Riyadh Muhaidat, Hussein M. Migdadi,Jamil N. Lahham and Ahmad Ali El-Oqlah

March 2013

Genotypic and phenotypic diversity among Bacillus species isolated from Mbuja, a Cameroonian traditional fermented condiment

  Dichotomous keys based on morphological, cultural and biochemical tests have long been used to identify Bacillus species. The analysis of 16S rDNA is suggested to be used for identification that is more exact. The present study was carried out to compare a conventional phenotypic method with the analysis of the 16S rRNA andgyrB gene for better identification, to determine their phylogenetic...

Author(s): Bouba-Adji Mohamadou, Carl Moses Mbofung and Georges Barbier

March 2013

Development of an RNA-interference procedure for gene knockdown in the poultry red mite, Dermanysus gallinae: Studies on histamine releasing factor and Cathepsin-D

  The poultry mite, Dermanyssus gallinae, is a parasite of fowl and birds worldwide, causing huge economic losses in production systems. We investigated gene-knockdown approach in identifying potential vaccine antigens, targeting Histamine Releasing Factor (HRF) and Cathepsin D (C), through immersion in dsRNA derived from the two genes, for 7 and 16 h respectively, alongside LacZ control....

Author(s): Lucy M. Kamau, Harry W. Wright, Alasdair J. Nisbet and Alan S. Bowman

March 2013

Genetic parameters and consequences of selection for short-term egg production traits in Japanese quail in a tropical environment

  The current study was conducted to investigate the effect of selection for short-term (30 days) egg production trait in Japanese quail (Coturnix coturnix  japonica) over three generations (Go, G1 and G2). Heritabilities were estimated using the mixed model least squares and maximum likelihood computer programme. Fertility percentages of 76.29, 70.06, and 65.98% and hatchability percentage...

Author(s): Okenyi, N., Ndofor-Foleng, H. M, Ogbu, C. C., and Agu, C. I.

March 2013

Pathogenicity of two seed-borne fungi commonly involved in maize seeds of eight districts of Azad Jammu and Kashmir, Pakistan

  This study was aimed to evaluate the pathogenicity of two mostly prevailing fungal species on maize (Zea maize L.) from different localities of Azad Jammu and Kashmir (AJK), Pakistan. Pathogenic fungi deteriorate food grains by producing mycotoxins and aflotoxins during storage consequently shedding menace on its nutritive quality. There were 12 species of mycoflora associated with...

Author(s):   Nazar Hussain⊃, Altaf Hussain⊃, Muhammad Ishtiaq⊃, Shehzad Azam and Tanveer Hussain    

March 2013

Growth promotion and protection against Orobanche foetida of chickpea (Cicer aerietinum) by two Rhizobium strains under greenhouse conditions

  Fetid broomrape (Orobanche foetida Poir.) is a chlorophyll lacking holoparasite that subsists on the roots of plants and causes significant damage to the culture of leguminous plants particularly chickpea (Cicer aerietinum L.). The investigation was done about potential of Rhizobium strains for biological control of O. foetida using a commercial chickpea...

Author(s): Imen Hemissi,, Yassine Mabrouk, Neila Abdi, Manel Bouraoui, Mouldi Saidi and Bouaziz Sifi

March 2013

Efficient regeneration of plants from shoot tip explants of Dendrobium densiflorum Lindl., a medicinal orchid

  Dendrobium densiflorum Lindl. is one of the horticulturally important orchids of Nepal due to its beautiful yellowish flower and medicinal properties. The present study was carried out for plant regeneration from shoot tip explants of D. densiflorum by tissue culture technique. The shoot tip explants of this species, obtained from in vitro grown seedlings were cultured on Murashige...

Author(s): Shreeti Pradhan, Yagya P. Paudel and Bijaya Pant

March 2013

Effectiveness of botanical powders against Callosobruchus maculatus (Coleoptera: Bruchidae) in some stored leguminous grains under laboratory conditions

  The study investigated the comparative efficacy of some botanicals (Aframomum melegueta   (K. M. Schumann) seed, Capsicum nigrum (L.) seed, Allium sativum(Woodwill) bulb, Zingiber officinale (Roscoe) rhizome, Azadiracta indica (A. Juss) leaves and Ocimum gratissimum (L.) leaves) and pirimiphos methyl powder in the suppression...

Author(s):   Felicia Nkechi Ekeh, Ikechukwu Eugene Onah, Chinedu Ifeanyi Atama, Njoku Ivoke, and Joseph Effiong Eyo  

March 2013

Growth and nitrogen metabolism changes in NaCl-stressed tobacco (Nicotiana rustica L. var. Souffi) seedlings

  While salinity effects have been largely documented in crop plants, little data are available on Nicotiana rustica species (snuff tobacco), mainly nitrogen metabolism changes. Here, tobacco (N. rustica L. var. Souffi) seedlings were grown for one month on control medium, and then exposed for seven days to different levels of salt stress (0, 50, 100 and 200 mM NaCl). A significant decrease...

Author(s): Chokri Zaghdoud, Houda Maâroufi-Dguimi, Youssef Ouni, Mokhtar Guerfel, Houda Gouia, Kamel-Eddine Negaz, Ali Ferchichi and Mohamed Debouba

March 2013

Effect of the fructose and glucose concentration on the rheological behavior of high fructose syrups

  The objective of this work was to study the effect of fructose and glucose content on the rheological behavior of syrups. Initially, high fructose syrup from the fructans present in leaves, bases and head of Agave tequilana Weber blue was obtained. Then, its contents of moisture, ash, fructose, glucose and direct and total reducing sugars were determined. Finally, the physicochemical properties of...

Author(s): José Luis Montañez-Soto, Luis Humberto González-Hernández, José Venegas-González, Aurea Bernardino Nicanor and Leopoldo González-Cruz

March 2013

Purification and characterization of protease enzyme from Burkholderia stabilis

  Burkholderia stabilis, a protease producing organism was isolated from green house soils in Chungchugnam-do province, Gongju-Gun area in South Korea. Optimum protease activity of 116.4 U/ml was observed in the growth medium containing 0.7% KH2PO4, 0.2% K2HPO4, 0.01% MgSO4.7H2O, 0.05% citric acid dehydrate, 0.1% yeast extract and 0.2% casein. The protease production was found to be optimized in 1: 5...

Author(s): Moon-Hyun Park, Buddhi Charana Walpola and  Min-Ho Yoon

March 2013

Cryopreservation of in vitro-grown shoot tips of apricot (Prunus armeniaca L.) using encapsulation-dehydration

  In vitro grown apricot (Prunus armeniaca L.) cv. El-Hamawey shoot tips were successfully cryopreserved using an encapsulation-dehydration procedure. Shoot tips were encapsulated in calcium-alginate beads before preculture on woody plant (WP) medium supplemented with different sucrose concentrations; 0.1, 0.3, 0.5, 0.75 and 1.0 M for 2, 4, 6 and 8 days at 25°C. Encapsulated shoot tips were...

Author(s): Hemaid I. A. Soliman

March 2013

Induction of apoptosis in human breast adenocarcinoma MCF-7 cells by tannic acid and resveratrol

  Tannic acid (TA), a plant polyphenol, has been described as having anticarcinogenic, antioxidant, antimutagenic, antimicrobial, antiallergic, antiinflammatory and astringent properties. Resveratrol (RES), a phytoalexin phenolic compound, has been known as an antioxidant, anticarcinogenic, antiinflammatory, antimutagenic, antiproliferative, antiviral, antibacterial, estrogenic and...

Author(s): Ahu Soyocak, Didem Turgut Cosan, Ayse Basaran, Hasan Veysi Gunes, Irfan Degirmenci and Fezan Sahin Mutlu

March 2013

In vitro influence of 2% chlorhexidine on links established at the hybrid layer between collagen fibers and nano adhesive used in the adhesive system

  In this study, 48 human teeth were chosen; they were extracted from natural sources. Sound dentin surfaces were exposed by operating low speed diamond disc at conventional speed with copious water spraying. The teeth were divided by randomizing technique, into two equal groups (n = 24): the control and the test group. Teeth in the control group followed the conventional method of etching, washing, applying...

Author(s): Cornel Gh. Boitor, Florin Stoica, Mihaela Cernusca-Mitariu, Mihai Burlibasaand Laura Stef

March 2013

Amount and qualities of carotenoids in fillets of fish species fed with natural feed in some fisheries of West African Coast

  Using column (CC), thin- layer (TLC) and high- performance liquid chromatography (HPLC), carotenoid content was examined in the fillets (muscles with skin) of 16 fish species from the fisheries of West African Coast. 15 carotenoids, including 6 ketocarotenoids (4’- hydroxyechinenone, canthaxanthin, phoenicopterone, phoenicoxanthin, astaxanthin, 7,8- didehydroastaxanthin) were...

Author(s): Bazyli Czeczuga, Janusz Semeniuk, Ewa Czeczuga-Semeniuk, Adrianna Semeniuk and Bernard Klyszejko

March 2013

Body extract of tail amputated zebrafish promotes culturing of primary fin cells from glass catfish

  The most spectacular regenerative events in vertebrates are epimorphic regeneration. In this study, interestingly, a whole-body extract 24 h after tail amputation enhanced primary cell growth and viability compared to that of a non-tail amputated body. Additionally, these effects of extract treatment in vitro were dose-dependent occurring at concentrations of 0.02, 0.05 and 0.1 mg/ml. This is the...

Author(s): Jee Eun Han, Ji Hyung Kim, Casiano Choresca, Sang Phil Shin, Jin Woo Junand Se Chang Park

March 2013

High genetic diversity of Mycospaherella graminicola (Zymoseptoria tritici) from a single wheat field in Tunisia as revealed by SSR markers

Microsatellite markers were used to assess genetic diversity of Mycosphaerella graminicola at a micro geographical scale in Tunisia. Forty five (45) isolates were sampled and assessed using seven pairs of single-locus microsatellite primers not previously tested on populations of M. graminicola in Tunisia. Genetic diversity of the field population ranged from 0.403 to 0.555 with an average of...

Author(s): Samia Berraies, Mohamed Salah Gharbi, François Belzile, Amor Yahyaoui, Mohamed Rebah Hajlaoui , Mokhtar Trifi , Martine Jean and Salah Rezgui