African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12227

Full Length Research Paper

Production and partial characterization of keratinase produced by a microorganism isolated from poultry processing plant wastewater

  Daniel M. T. Tapia* and Maria Lucia Garcia Simões      
  Departamento de Engenharia Agricola e Solos - DEAS, Universidade Estadual do Sudoete da Bahia-UESB, Estrada do Bem Querer Km 4, Vitoria da Conquista Bahia- Brazil.  
Email: [email protected]

  •  Accepted: 16 October 2007
  •  Published: 05 February 2008

Abstract

 

Streptomyces was isolated from poultry plant wastewater, showed high keratinolytic activity when cultured on feather meal medium. Optimum keratinolytic activity was observed at 40oC and pH 8.0. The enzyme also showed to be stable between 40 and 60oC. The keratinolytic activity was not inhibited by EDTA, DMSO and Tween 80. On the other hand, CaCl2, ZnCl2, and BaClslightly inhibited the keratinolytic activity. The Streptomyces isolated might be useful in leather, keratin waste treatment, animal feeding industry, and also cosmetic industry.

 

Key words: Keratinase, streptomyces, wastewater.