African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12487

Short Communication

Studies on isolation and partial purification of lysozyme from egg white of the lovebird (Agapornis species)

R. Saravanan1*, A. Shanmugam1, Preethi Ashok2, D. Sathish Kumar2, K.  Anand3, Amitesh Suman4 and Fredrick Robin Devadoss5
1Centre of Advanced Study in Marine Biology, Annamalai University, Parangipettai-608 502, Tamil Nadu, India. 2Department of Biotechnology, SRM Arts & Science College, Kattankulathur-603 203, Tamil Nadu, India. 3Loyola College, Chennai-600 034, Tamil Nadu, India. 4Department of Biotechnology, Amity Institute of Biotechnology, Noida. 5Department of Physics, The American College, Madurai – 625002, India.
Email: [email protected]

  •  Accepted: 12 June 2007
  •  Published: 05 January 2009

Abstract

Lysozyme was extracted from the egg white of the eggs of lovebird (Agapornis sp.) and partially purified using the dialysis membrane. The amount of lysozyme extracted was determined through absorbance assays using Micrococcus lysodeikticus cell walls as substrate. The total protein content in the crude (0.5 mg/ml) and partially purified (0.01 mg/ml) enzyme extract was estimated. The presence of lysozyme enzyme in the egg white was confirmed and its molecular weight (14.3 kDa) was determined through SDS-PAGE.

 

Key words: Lysozyme, Agapornis, Micrococcus lysodeikticus, SDS-PAGE.

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This article is published under the terms of the Creative Commons Attribution License 4.0

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