African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12487

Full Length Research Paper

Improved secretory production of calf prochymosin by codon optimization and disruption of PMR1 in Kluyveromyces lactis GG799

Zhen Feng1*, Jing Ren1, Yanhe Zhang2 and Sen Hu2
  1College of Food Science, Northeast Agricultural University, 59 Mucai Road, 150030 Harbin, Heilongjiang, People’s Republic of China. 2Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, 427 Maduan Road, 150001 Harbin, Heilongjiang, People’s Republic of China.
Email: [email protected]

  •  Accepted: 05 August 2011
  •  Published: 12 September 2011

Abstract

 

Chymosin as an important industrial enzyme used widely in cheese manufacture. In our preliminary study, low yields (80 U mL-1) were obtained when Kluyveromyces lactis GG799 was used to express chymosin. We investigated whether this poor secretion could be improved by codon optimization and disruption of PMR1 gene inK. lactis GG799. Our study shows that combination of codon optimization and disruption of the PMR1 gene resulted in secretion level of chymosin that was increased 10-fold. Our results show that codon optimization and disruption of thePMR1 gene synergistically stimulate the secretion of calf prochymosin in K. lactisGG799.

 

Key words: Kluyveromyces lactis, codon optimization, PMR1, prochymosin.

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This article is published under the terms of the Creative Commons Attribution License 4.0

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