African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12500

Short Communication

Rapid detection of methicillin-resistant staphylococci by multiplex PCR

  Mohammad Reza Safari Motlagh1 and Maesumeh Anvari2*  
1Department of Plant Pathology, Faculty of Agriculture, Islamic Azad University, Rasht Branch, Rasht, Iran. 2Department of Microbiology, Faculty of sciences, Islamic Azad University, Rasht Branch, Rasht, Iran.
Email: [email protected]

  •  Accepted: 25 June 2010
  •  Published: 08 November 2010

Abstract

 

A rapid and sensitive method for excluding the presence of methicillin-resistantStaphylococcus aureus (MRSA) in clinical samples was developed. The combination of MRSA detection by mecA coaA PCR with prior enrichment in selective broth was tested for 300 swabs. PCR identified 26 MRSA-positive samples, in concordance with conventional methods.

 

Key words: Staphylococcus aureus, swabs, mecA coaA.

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This article is published under the terms of the Creative Commons Attribution License 4.0

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