African Journal of

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12481

Full Length Research Paper

In vitro plant regeneration from protocorms-like bodies (PLBs) and callus of Phalaenopsis gigantea (Epidendroideae: Orchidaceae)

A. Niknejad1*, M. A. Kadir1* and S. B. Kadzimin2
  1Department of Agriculture Technology, Faculty of Agriculture, University Putra Malaysia, Malaysia. 2Department of Crop Science, Faculty of Agriculture, University Putra Malaysia, Malaysia.
Email: [email protected], [email protected]

  •  Accepted: 20 May 2011
  •  Published: 26 September 2011



Phalaenopsis, with long arching sprays of flowers, are among the most beautiful flowers in the world. Phalaenopsis is an important genus and one of the most popular epiphytic monopodial orchids, grown commercially for the production of cut flowers and potted plants. Most of them have different and interesting morphological characteristics which have different value to the breeders. Phalaenopsis gigantea is one of the most difficult to grow and has the potential of producing beautiful hybrids. An efficient and reproducible method for large-scale propagation of Ph. giganteausing leaf sections has been developed. Leaf sections from in vitro young plants were cultured on New Dogashima Medium (NDM) supplemented with cytokinins (6-Benzylaminopurine (BAP), Thidiazuron (TDZ), and Kinetin (KIN), each at 0.01, 0.1, 0.5 and 1.0 mg/L) alone and in combinations with (auxins a-naphthaleneacetic acid (NAA), at 0.01, 0.1, 0.5 and 1.0 mg/L). The explants developed calli and protocorm-like-bodies (PLBs) within 6 weeks of culture. Treatment TDZ in combination with auxins was found to be the best for the induction of callus and PLBs. In vitroregeneration of Ph. gigantea PLB was achieved by exposure to light and transferring to hormone free NDM solid medium.


Key words: Phalaenopsis, PLBs, new Dogashima medium, regeneration.


BAP, 6-Benzylaminopurine; CW, Coconut Water; KIN, Kinetin; MS,Murashige and Skoog; NAA, naphthalene Acetic Acid; NDM, New Dogashima Medium; VW, Vacin and Went; TDZ, Thidiazuran.