African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12487

Full Length Research Paper

Chromium stress in Brassica juncea L. cv. 'Pusa Jai Kissan' under hydroponic culture

Rehana Hamid1, Javid A. Parray2*, Azra N. Kamili2 and Mahmooduzzafar1
  1Department of Botany, Jamia Hamdard (Deemed University), Hamdard Nagar, New Delhi-110062, India. 2Department of Environmental Science/Centre of Research for Development, University of Kashmir-Srinagar-Jand K, India-190006.
Email: [email protected]

  •  Accepted: 28 June 2012
  •  Published: 08 November 2012

Abstract

 

Chromium (Cr) entering plant tissue inhibits most physiological processes at all levels of metabolism including inhibition of growth, photosynthesis and nitrate assimilation. Since Cr exists in many forms, its toxicity to plants depends on its valence state, with Cr (VI) found to be highly toxic and mobile than Cr (III). Different concentrations of Cr (0, 25, 50 and 100 µM) in the form of K2Cr2Owas added to 30 days old Brassicajuncea plant and harvested on the 3rd and 5th days after treatment for estimation of plant growth, chlorophyll, total soluble protein, free amino acids and nitrate reductase activity. Cr was found to cause deleterious effects on whole plant growth. The potential of plants with the capacity to accumulate or to stabilize Cr compounds for bioremediation of Cr contamination has gained interest in recent years. The biochemical aspects like photosynthetic pigments (Chl a and Chl b), total protein and amino acids content decreased with Cr concentration. A significant increase in nitrate reductase activity was observed corresponding to Cr concentration.

 

Key words: Brassica juncea, chromium, heavy metal, phytoremediation

Abbreviation

Cr, Chromium; DAT, days after treatment; NR, nitrate reductase.