African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12485

Full Length Research Paper

Rhodococcus opacus strain RW, a resorcinol-degrading bacterium from the gut of Macrotermes michaelseni

David Kamanda Ngugi1Y, Muniru Khamis Tsanuo2 and Hamadi Iddi Boga1*
1Botany Department, and 2Chemistry Department, Jomo Kenyatta University of Agriculture and Technology, P.O. Box 62000 (00200) Nairobi, Kenya.
Email: [email protected]

  •  Accepted: 14 May 2005
  •  Published: 31 July 2005

Abstract

 

The population of resorcinol degrading bacteria in the intestinal tract of fungi-cultivating termite, Macrotermes michaelseni, was estimated to be 6.8 × 102cells/ml. A gram-positive bacterium designated RW, capable of degrading resorcinol, was isolated from the highest positive dilution. Isolate RW could also degrade phenol and benzoic acid aerobically, and anaerobically using nitrate as an electron acceptor. The isolate is a rod-shaped bacterium that exhibited evolutionary relatedness with the genus Rhodococcus, as determined by phenotypic traits and physiological tests, and a 16S rRNA gene sequence similarity value of 99.6% to the closest Rhodococcus opacus strain. On the basis of these results isolate RW is proposed as a new strain in the species R. opacus. The ability of the isolate to degrade resorcinol, phenol and benzoic acid makes it a potential candidate for use in bioremediation of environments contaminated by such or related compounds.

 

Key words: Rhodococcus opacus, resorcinol, Macrotermes michaelseni, termites, bioremediation, monoaromatic compounds.