African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12227

Full Length Research Paper

Effect of collection time on the viability of banana pollen grains

Taliane Leila Soares*
  • Taliane Leila Soares*
  • Embrapa Cassava and Fruits, Brazilian Agricultural Research Corporation, Cruz das Almas, BA, 44380-000, Brazil.
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Everton Hilo de Souza*
  • Everton Hilo de Souza*
  • Embrapa Cassava and Fruits, Brazilian Agricultural Research Corporation, Cruz das Almas, BA, 44380-000, Brazil.
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Laecio Fernandes Souza Sampaio
  • Laecio Fernandes Souza Sampaio
  • Center of Agricultural, Environmental and Biological Sciences, Federal University of Reconcavo da Bahia, Cruz das Almas, BA, 44380-000, Brazil.
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Angelica Pereira de Carvalho Costa
  • Angelica Pereira de Carvalho Costa
  • Center of Agricultural, Environmental and Biological Sciences, Federal University of Reconcavo da Bahia, Cruz das Almas, BA, 44380-000, Brazil.
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Sebastiao de Oliveira e Silva
  • Sebastiao de Oliveira e Silva
  • Center of Agricultural, Environmental and Biological Sciences, Federal University of Reconcavo da Bahia, Cruz das Almas, BA, 44380-000, Brazil.
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Janay Almeida dos Santos-Serejo
  • Janay Almeida dos Santos-Serejo
  • Embrapa Cassava and Fruits, Brazilian Agricultural Research Corporation, Cruz das Almas, BA, 44380-000, Brazil.
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  •  Received: 27 January 2015
  •  Accepted: 30 March 2015
  •  Published: 08 April 2015

Abstract

The objective of this study was to assess the pollen viability of six improved diploid banana plants (AA) collected in different periods of the day, in two seasons of the year (winter and summer), using in vitro germination test and staining. Pollen grains collected at 8 am (anthesis), 10 am, 12 pm, 2 pm and 4 pm were evaluated. We used a culture medium for pollen germination containing 15% sucrose, 0.01% H3BO3, 0.01% KNO3, 0.03% Ca(NO3)2.4H2O, and 0.02% MgSO4.7H2O, solidified with 0.8% agar, adjusted to different pH. The pollen viability was evaluated by staining with 2,3,5-triphenyltetrazolium chloride (TTC). The highest pollen germination rates and viability were obtained at 8 am and the lowest at 4 pm, in both seasons. The average in vitro germination percentage and viability level were negatively influenced by the number of hours after anthesis. During the summer, the pollen viability and in vitro germination rates were highest when compared to winter period. The results presented can help at the selection of genetic materials and enable inferences on the best pollen collection time for use in cross breeding programs for plant improvement.

 

Key words: Musa spp., in vitro pollen germination, 2,3,5-triphenyltetrazolium chloride.

Abbreviation

TTC, 2,3,5-Triphenyltetrazolium chloride; RH, relative humidity; H3BO3, boric acid; KNO3, potassium nitrate; Ca(NO3)2.4H2O, calcium nitrate tetrahydrate; MgSO4.7H2O, magnesium sulfate heptahydrate; HCl, hydrochloric acid.