African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12294

Table of Content: 8 April, 2015; 14(14)

April 2015

First detection of bla TEM, SHV and CTX-M among Gram negative bacilli exhibiting extended spectrum β-lactamase phenotype isolated at University Hospital Center, Yalgado Ouedraogo, Ouagadougou, Burkina Faso

Resistance to a wide variety of common antimicrobials is observed among clinical strains designed as extended spectrum β-lactmase (ESBL) producers. They produce enzymatic protein which inactivates efficiently oxyimino cephalosporin and constitutes a serious global health concern that has complicated treatment strategies. Many studies report high prevalence of ESBL producers among Gram negative bacilli. The aim of...

Author(s): K. J. Zongo, A. Metuor Dabire, L. G. Compaore, I. Sanou, L. Sangare, J. Simpore and B. Zeba

April 2015

Detection of Pseudomonas fluorescens from broth, water and infected tissues by loop-mediated isothermal amplification (LAMP) method

Loop mediated isothermal amplification is rapid, highly sensitive and specifically developed method for detection of bacterial infections. AprX gene for alkaline metalloprotease of Pseudomonas fluorescens was used to design four primers and loop mediated isothermal amplification (LAMP) conditions were standardized for amplification of DNA. LAMP primers successfully amplified P. fluorescens from DNA and bacterial cells...

Author(s): Pooja Saharan, Joginder S. Duhan and Suresh K. Gahlawat

April 2015

Detection of novel polymorphisms in the mitochondrial DNA D-Loop hypervariable region HVI from 400 healthy unrelated individuals from central and North-central Iraq

The mitochondrial DNA (mtDNA) is a small circular genome located within the mitochondria in the cytoplasm of the cell and a smaller 1.2 kilobase pair fragment, called the control region (D-loop). The aims of this research were to study this region by using the Sanger sequencing technique and establish the degree of variation characteristic of a fragment. FTA® Technology (FTA™ paper DNA extraction) was utilized...

Author(s): Ameera, Omran Hussein, Imad, Hadi Hameed and Muhanned, Abdulhasan Kareem

April 2015

Oil palm fruit fibre promotes the yield and quality of Lentinus squarrosulus (Mont.) Singer, an edible Nigerian mushroom

Agricultural production and the agro-food industry furnish large volumes of solid wastes, which when unutilized could lead to environmental pollution. An attempt was made to utilize wastes from the oil palm and timber industries for the cultivation of Lentinus squarrosulus, a Nigerian edible mushroom. Mahogany sawdust (MSD), Gmelina sawdust (GSD), oil palm fruit fibre (OPFF) and oil palm empty fruit bunch (OPEFB)...

Author(s): Chiejina, Nneka Virginia and Osibe, Dandy Ahamefula,

April 2015

Catalase activity of cassava (Manihot esculenta) plant under African cassava mosaic virus infection in Cape coast, Ghana

African cassava mosaic virus has caused an immersed low yield of the cassava crop. The virus impacts stress on the cellular metabolism of the plant producing a lot of reactive oxygen species and increases the expression of the antioxidant enzymes. The activity of catalase as a response to oxidative stress was investigated in this research. Cassava leaf extract infected with the African cassava mosaic virus and...

Author(s): Sabastina Amoako, Adams Yahaya and Justice Kwabena Sarfo

April 2015

Effect of collection time on the viability of banana pollen grains

The objective of this study was to assess the pollen viability of six improved diploid banana plants (AA) collected in different periods of the day, in two seasons of the year (winter and summer), using in vitro germination test and staining. Pollen grains collected at 8 am (anthesis), 10 am, 12 pm, 2 pm and 4 pm were evaluated. We used a culture medium for pollen germination containing 15% sucrose, 0.01% H3BO3, 0.01%...

Author(s): Taliane Leila Soares, Everton Hilo de Souza, Laecio Fernandes Souza Sampaio, Maria Angélica Pereira de Carvalho Costa, Sebastião de Oliveira e Silva and Janay Almeida dos Santos-Serejo

April 2015

Bioprospecting of yeasts for amylase production in solid state fermentation and evaluation of the catalytic properties of enzymatic extracts

Profiling microorganisms with potential for amylase production in low cost culture media has been widely recognized due to its broad applicability. The aim of this study was to select yeast strains with potential to produce amylolytic enzymes by solid state fermentation. Fifty-four (54) strains were assessed and three exhibited ability to produce amylases: Candida parapsilosis with 14.68 U/mL (146.8 U/g substrate);...

Author(s): Ana Paula Aguero de Oliveira, Maria Alice Silvestre, Heloíza Ferreira Alves-Prado, André Rodrigues, Marcelo Fossa da Paz, Gustavo Graciano Fonseca and Rodrigo Simões Ribeiro Leite

April 2015

Antibacterial activity of watermelon (Citrullus lanatus) seed against selected microorganisms

This study was aimed at evaluating the effect of extraction methods on the antibacterial activity of Citrullus lanatus seed extract. C. lanatus (watermelon) is a popular fruit consumed all over the world. Three solvents were used for the extraction process: chloroform, methanol and distilled water while two extraction conditions- cold extraction and Soxhlet extraction (coded as hot in this study) were employed....

Author(s): Adelani-Akande Tabitha Adunola, Ajiba Lilian Chidimma, Dahunsi Samuel Olatunde and Oluyori Abimbola Peter

April 2015

Chemical characterization of passion fruit (Passiflora edulis f. flavicarpa) seeds

The aim of this study was to determine the chemical characteristics of passion fruit seeds and their oil for possible use in human food and reduction of organic waste from fruit industrialization. Passion fruit seeds were analyzed for moisture, lipids, proteins, ash, fibers, titratable acidity, pH, soluble solids and antioxidant activity. The oil was characterized for parameters such as acid value, saponification,...

Author(s): Silva, R. M., Placido, G. R., Silva, M. A. P., Castro, C. F. S., Lima, M. S. and Caliari, M.

April 2015

Mycosynthesis of iron nanoparticles by Alternaria alternata and its antibacterial activity

Nanotechnology is one of the most emerging fields in the recent years. In the current investigation, we report the biosynthesis of iron nanoparticles (Fe-NPs) employing Alternaria alternata fungus, which is an eco-friendly process for the synthesis of metallic nanoparticles. Fe-NPs were synthesized through the reduction of aqueous Fe3+ ion in the dark conditions. Ultraviolet–visible spectrum of the...

Author(s): Mohamed, Y. M., Azzam, A. M., Amin, B. H. and Safwat, N. A.

April 2015

Physicochemical parameters and antibiotics residuals in Algerian honey

The aim of the present study was to evaluate the quality of 36 samples of different honey type supplied by local producers from Algeria in order to verify its compliance with the standards of Codex Alimentarius and European Union (EU). For that, five physicochemical parameters were analyzed using the HPLC method: hydroxyl-methyl furfural (HMF), sugars, diastase activity and search of antibiotic contamination with...

Author(s): Radia DRAIAIA, Azzedine CHEFROUR, Nicoletta DAINESE, Alice BORIN, Chiara MANZINELLO , Albino GALLINA and Franco MUTINELLI