African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12491

Full Length Research Paper

Differential expression of cytochrome P450 genes in a laboratory selected Anopheles arabiensis colony

Givemore Munhenga1,2 and Lizette L. Koekemoer1,3*
1Vector Control Reference Unit , National Institute for Communicable Diseases, NHLS, Private Bag X4, Sandringham, Johannesburg 2131, South Africa. 2School of Animal, Plant and Environmental Sciences, University of the Witwatersrand, Johannesburg, South Africa. 3Malaria Entomology Research Unit, School of Pathology, Faculty of Health Sciences, University of the Witwatersrand, Johannesburg, South Africa.
Email: [email protected], [email protected]

  •  Accepted: 22 August 2011
  •  Published: 03 October 2011

Abstract

In southern Africa pyrethroid, resistance in Anopheles arabiensis is mainly mediated by cytochrome P450s. The spectra of P450 genes involved are not fully understood. We report on the transcriptional profile of six P450 genes previously implicated in pyrethroid resistance from a laboratory selected permethrin-resistance.Quantification of expression levels of CYP6Z1, CYP6Z2, CYP6Z3, CYP6M2, CYP6P3 and CYP4G16 was performed using qPCR from a susceptible and permethrin resistant selected colony. CYP6Z1, CYP6Z2 and CYP6M2 were significantly up-regulated in the selected colony with a relative fold over expression of 4.7, 1.7 and 1.4 respectively. Increase in expression levels of three genes in the selected strains suggests their roles in permethrin metabolism. These results provide useful information on future studies to develop new insecticides and tools for detecting and managing insecticide resistance.

 

Key words: KwaZulu-Natal, Anopheles arabiensis, pyrethroid resistance, cytochrome P450, synergist

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This article is published under the terms of the Creative Commons Attribution License 4.0

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