African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12487

Full Length Research Paper

Pollen morphology and variability of Tulipa hungarica Borb

Dušica Ćalić*, Nina Devrnja, Jelena Milojević, Snežana Zdravković-Korać, Ljiljana Tubić, Milutin S. Djuričković and Branka Vinterhalter  
Department of Plant Physiolgy, Institute for Biological Research “Siniša Stanković“, University of Belgrade, Serbia.
Email: [email protected]

  •  Accepted: 01 December 2011
  •  Published: 10 January 2012

Abstract

Pollen morphology of the endemic species Tulipa hungarica Borb was investigated with the goal of diversity protection and conservation. Morphology of pollen was evaluated using a scanning electron microscopy. Pollen grain was monosulcate with perforate striate exine surface. Also, pollen of T. hungarica showed differences in size, shape, and staining intensity after aceto-orceine treatment. The smallest aceto-orcein treated microspore had a diameter of 13.65 μm, while the largest had a diameter of 60.15 μm. 4,6-Diamidino-2-phenylindole (DAPI) staining showed that fifty percent of investigated microspores had one nucleus, and the other half had two nuclei. A rapid method with fluorescein diacetate – FDA was used to determine pollen viability. Up to (80%) of analyzed microspores showed high viability.

 

Key words: Microspore size, pollen grain, pollen viability, staining intensity, Tulipa.

Copyright © 2024 Author(s) retain the copyright of this article.

This article is published under the terms of the Creative Commons Attribution License 4.0

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