January 2012
cDNA sequence and tissue expression analysis of glucokinase from liver of grass carp (Ctenopharyngodon idella)
A full-length cDNA coding glucokinase (GK) was cloned from the liver of grass carp (Ctenopharyngodon idella) by reverse transcriptase-polymerase chain reaction (RT-PCR) and rapid amplification of cDNA ends methods. The cDNA obtained was 2066 bp exclusive of poly (A) residues with a 1431 bp open reading frame encoding 476 amino acids. The GK protein has a calculated molecular...
January 2012
Cloning and expression of porcine SRPK1 gene
Protein SRPK1 acts as a crucial element in the pre-initiation complex of transcription, which play an important role in the regulation procession of gene expression. This study was carried out in order to explore the genetic characteristic of SRPK1 in pigs.SRPK1 gene came from Yorkshire, a pig that was cloned by real time polymerase chain reaction (RT-PCR), yet coding sequence and partial...
January 2012
Molecular cloning and expression analysis of an Mn-superoxide dismutase gene in sugarcane
Superoxide dismutases (SODs) play an important role in stress-tolerance in plants. In this study, for the first time, a full-length cDNA sequence of MnSOD gene, termed asSc-MnSOD (GenBank accession number: GQ246460), was obtained in sugarcane. Sequence analysis revealed that Sc-MnSOD gene was 919 bp long, including a 702 bp ORF, the 5’ UTR of 99 bp and 3’UTR of 118 bp. It...
January 2012
Using innovation platforms to scale out soil acidity-ameliorating technologies in Dedza district in central Malawi
Soil acidity is a serious constraint in crop production in some parts of Malawi, particularly in high rainfall and high altitude areas. A learning platform was established in Bembeke Extension Planning Area (EPA) (14° 21′ E and 34° 21′ S, 1650 masl, normal annual rainfall of 1300 to 1500 mm) in Dedza District to scale out lime applications as amendments to low pH soils. In 2006/2007 and...
January 2012
Identification and characterization of a Bacillus subtilis strain TS06 as bio-control agent of strawberry replant disease (Fusarium and Verticilium wilts)
Replant disease is a major limitation for strawberry production in greenhouse. Bio-control may be a good way to cope with the replant diseases. Here, we report identification and characterization of a bacterial strain TS06 that may be used as a bio-control agent against the replant diseases in strawberry. TS06 was identified as a new strain of Bacillus subtilis based on the homology of its 16S rRNA...
January 2012
Cloning and expression analysis of a LFY homologous gene in Chinese jujube (Ziziphus jujube Mill.)
LEAFY (LFY) homologous genes are necessary for the transition from vegetative to reproductive development in flowering plants. The full-length cDNA of a LFY homologwas successfully isolated from floral buds of Chinese Jujube (Ziziphus jujube Mill.) by degenerate reverse transcriptase-polymerase chain reaction (RT-PCR), rapid amplification of cDNA ends (RACE), and Blastn analysis....
January 2012
Microbial activity of soil during the inoculation of soya bean with symbiotic and free-living nitrogen-fixing bacteria
Soya bean has a great importance in the organic production due to its ability to coexist with nitrogen-fixing bacteria of the genus Bradyrhizobium. The soya bean seed inoculated with the symbiotic nitrogen-fixing bacteria Bradyrhizobium japonicum and with a mixture of nitrogen-fixing bacteria was observed in this study. The following highly efficient strains of free nitrogen-fixing...
January 2012
Regenerability of elite tropical maize (Zea mays L.) inbred lines using immature zygotic embryo explants
Five elite tropical maize inbred lines; CML395, CML443, CML442, MAS [MSR/312]-117-2-2-1-B-5-B) and CML216 as a control, were evaluated for their regenerability making use of calli derived from immature zygotic embryos. Murashige and Skoog basal salts supplemented with 1.0, 1.5, 2.0 and 2.5 mg/L 2,4-dichlorophenoxyacetic acid were used to induce callus. Callus induction frequency and formation of embryogenic callus...
January 2012
Silicon mediated biochemical changes in wheat under salinized and non-salinzed solution cultures
Silicon (Si) can alleviate salinity damage, a major threat to agriculture that causes instability in wheat production. We report on the effects of silicon (150 mg L-1) on the morphological, physiological and biochemical traits in wheat (Triticum aestivum L.) cultivars (salt sensitive; Auqab-2000 and salt tolerant; SARC-5) differing in salt tolerance under saline (10 dS m-1) and non-saline (2 dS m-1) hydroponic...
January 2012
Pollen morphology and variability of Tulipa hungarica Borb
Pollen morphology of the endemic species Tulipa hungarica Borb was investigated with the goal of diversity protection and conservation. Morphology of pollen was evaluated using a scanning electron microscopy. Pollen grain was monosulcate with perforate striate exine surface. Also, pollen of T. hungarica showed differences in size, shape, and staining intensity after aceto-orceine treatment. The...
January 2012
Influence of salicylic acid on growth and some biochemical parameters in a C4 plant (Panicum miliaceum L.) under saline conditions
Salt stress is considered as a restricting factor for plant products. Therefore, many compounds were applied to minimize the harmful effects of this stress. In this study, millet plants (Isfahan cultivar) were sprayed with 0.1, 0.3, 0.5 and 1 mM salicylic acid and then treated with 0, 150, 225 and 325 mM NaCl. Our results show that in plants not sprayed with salicylic acid but treated with NaCl,...
January 2012
Heavy metal ions adsorption by suspended particle and sediment of the Chalus River, Iran
Nowadays, it is important to evaluate the self-purifying capacity of rivers because of the different kinds of pollutants discharged into them. Important kind of pollutants and heavy metals exist in wastewaters industries. When the Sorb Dona mine is placed in Upper Chalus River, in the west of Mazandaran, products of mine wastewater are discharged into the river, making this important and protective river to...
January 2012
Antioxidative enzyme activities in the leaves and callus tissues of salt-tolerant and salt-susceptible melon varieties under salinity
The response of the antioxidant system to salt stress was studied in the leaves and callus tissues of 4 Turkish melon varieties (Cucumis melo L.) Besni, Yuva, Midyat, and Semame and a melon cultivar Galia C8. The antioxidant capability of the plants was determined by measuring superoxide dismutase (SOD) and catalase (CAT) activities. On the 8th day of the salt stressed callus culture, growth...
January 2012
Augmenting the salt tolerance in wheat (Triticum aestivum) through exogenously applied silicon
Although silicon improves the salt tolerance in grasses, the mechanism involved is still ambiguous. Moreover in wheat, classified as Si-accumulator, silicon can alleviate the salt stress. Hence in this study, the effects of silicon using calcium silicate (150 mg/L) on the morphology, physiology and biochemistry of wheat genotypes (salt sensitive; Auqab-2000 and salt tolerant; SARC-5) differing in salt...
January 2012
Degradation of petroleum hydrocarbons by oil field isolated bacterial consortium
A mixed consortium was prepared with 15 bacteria isolated by enrichment technique from the sample collected from an oil contaminated site. This consortium was incubated with crude oil to investigate the metabolic capability of bacteria. The degradation efficiency of the isolates in consortium was checked with 2% crude oil by shake flask transformation in mineral salt medium, at 37°C for 24 days. Total...
January 2012
Growth of Enterococcus durans E204 producing bacteriocin-like substance in MRS Broth: Description of the growth and quantification of the bacteriocin-like substance
Bacteriocin-like substance E204 is an antimicrobial compound produced byEnterococcus durans E204 isolated from camel milk of Morocco that shows a broad spectrum of inhibitory activity against taxonomically related microorganisms. It is sensitive to digestive proteases. In the first study, de Man, Regosa and Sharpe (MRS) broth was inoculated by E204 strain, incubated for 30°C at 200 rpm and monitored...
January 2012
Biological activity of Streptomyces noursei against ochratoxin A producing Aspergillus niger
Aspergillus niger isolated from some common medicinal and herbal plants used in Egypt, was found to be producer for ochratoxin A. This research investigate the biological control of ochratoxin A production by A. niger, using some bacterial isolates belonging to true bacteria (Bacillus pumilus), Lactic acid bacteria (Lactobacillus acidophilus, Lactobacillus...
January 2012
Peroxidase isozyme profiles in some sweet cherry rootstocks and ‘0900 Ziraat’ cherry variety
This study was carried out on one- year old trees of ‘0900 Ziraat’ variety grafted onto KuÅŸ kirazı, Kara idris, Sarı idris, MaxMa 14, MaxMa 60 and Gisela 5 in order to determine their compatibility. For this purpose, peroxidase isoenzyme bands were determined with polyacrylamide gel electrophoresis (PAGE). Saplings were grafted at the beginning of September with ‘T’ budding....
January 2012
Rapid detection and isolation of Salmonella sp. from amphibians and reptiles
The principal objective of this study was to assess the presence of Salmonella sp. in imported amphibians and reptiles. A total of 49 samples were collected from commercial pet shops and life science museums in Korea and examined via the traditional detection method (TDM) and the PCR method. Salmonella typhimuriumand Salmonella enteritidis were screened via multiplex PCR (mPCR). The...
January 2012
Evidence for a role of KISS-1/GPR54 system in decreased luteinizing hormone (LH) secretion in fasted prepubertal ewes
Short fasting has been shown to suppress luteinizing hormone (LH) secretion in ewes, however, the molecular mechanism underlying this phenomenon is not clear. Based on recent discovery of the crucial role of KiSS-1/GPR54 system in the central control of the GnRH axis, we hypothesized that KiSS-1/GPR54 system was involvedin the inhibitory effect of fasting on LH secretion in ewes. To test...
January 2012
Characterization of polycaprolactone and starch blends for potential application within the biomaterials field
Thermally blending starch and polycaprolactone with the compatibilizer methylenediphenyl diisocyanate (MDI) distributed in the polyester phase had been previously confirmed to enhance the mechanical properties and interface between the two phases of the blends. In this study, blends of polycaprolactone with various levels of starch and 1.0% MDI were prepared through melt blending using a twin-screw...
January 2012
Primary drug resistance patterns in newly diagnosed tuberculosis patients in Yazd, Southern Province of Iran
Prevalence of multidrug resistant (MDR) and extensively drug-resistant tuberculosis (XDR) has been a cause of concern for tuberculosis (TB) control in both developed and developing countries. The objective of this study was to investigate the prevalence of drug resistance patterns among newly diagnosed tuberculosis cases, in Yazd, the southern province of Iran, during 2009 to 2010. The drug susceptibility...
January 2012
Effects of RNA interference targeting Smad7 on nerve cells ischemic injury induced in PC12 cells
Ischemic cerebrovascular disease is a global health problem. According to the World Health Organization, ischemic stroke is actually the most common cause of death in the world. ActA/smads signaling pathways was shown to be required for the differentiation-associated physiological apoptosis of stroke. Although smad7 is an important regulator of ActA/smads signaling via a negative feedback...
January 2012
The phenomenon of cross tolerance in osmotically and ionically adapted rice (Oryza sativa L.) cell lines
In the present study, cell lines of local rice (Oryza sativa L.) cv. Swat-1 were incrementally adapted to osmotic [20% Poly ethylene glycol (PEG)] and ion specific (20 mM LiCl) stresses to investigate the phenomenon of cross tolerance. The tolerances of adapted lines were stable up to six generation on stress free medium. Both adapted cell lines were highly tolerant to either osmotic or ionic...
January 2012
Serum biochemical values and mineral contents of tissues in Przewalski’s and Tibetan gazelles
Reference values were established for some serum hematological and biochemical constituents in Przewaiski’s and Tibetan gazelles in China. The contents of mineral elements in the blood, hair, liver, lung and muscle of Przewaiski’s and Tibetan gazelles were also measured. All values are reported for the first time for Przewaiski’s and Tibetan gazelles. Most hematological...
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