African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12294

Full Length Research Paper

Study on candidate gene for fecundity traits in Xingjiang Cele black sheep

Hongcai Shi1,2*, Jie Bai2,3, Zhigang Niu2, Muniresha4, Lijun Fen1,2 and Bin Jia1  
  1College of Animal Science and Technology, Shihezi University, Shihezi 832000, China. 2Key Laboratory of Livestock Reproduction and Breed Biotechnology of Ministry of Agriculture, Urumqi 830000, China. 3College of Life Science and Technology, Xinjiang University, Urumqi 830000, China. 4Bureau of Husbandry and Veterinary, Cele, Xingjiang 848300, China.  
Email: [email protected]

  •  Accepted: 30 September 2010
  •  Published: 06 December 2010

Abstract

 

The aim of the present study is to find a potential candidate gene for high fecundity in Cele black sheep. Polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) technology was used to detect single nuclear polymorphism (SNP) of four candidate genes (BMPR-IBBMP15, GDF9, andESRα) in Cele black sheep. The results showed that (i) A-G mutation was found at 746 bp in BMPR-IB in which the frequencies of homozygote (BB), heterozygote (B+) and wild type (++) were 0.113, 0.471, and 0.416, respectively. Significant differences were observed in litter size between ++ and B+ (P < 0.01) and between ++ and BB of individuals (P < 0.05). (ii) C-G mutation was found at exon 1 of ESRα in which the frequencies of homozygote, heterozygote and wild type were 0.047, 0.321 and 0.631, respectively. No significant difference was observed in litter size among the genotypes of ESRα (P > 0.05). (iii) No polymorphism was found in four mutation sites (FecXG, FecXB, FecXI, FecXH) of BMP15 and in one mutation site (FecGH) of GDF9 gene. The results indicate that fecundity characteristic was positively correlated to BMPR-IB. However, there was no relation between fecundity characteristic and detected SNP sites of ESRαBMP15 and GDF9 genes. These preliminary results showed that the BMPR-IB gene is either a major gene that influences the prolificacy in Cele black sheep or a molecular genetic marker in close linkage with such a gene.

 

Key words: Cele black sheep, fecundity candidate gene, BMPR-IB, BMP15, GDF9,ESRα.

Abbreviation

Abbreviations: SNP, Single nuclear polymorphism; PCR-RFLP, polymerase chain reaction-restriction fragment length polymorphism; BB, homozygote; B+,heterozygote; ++, wild type.