Full Length Research Paper
Abstract
Terbutaline (β2-adrenergic agonist) can increase alveolar fluid clearance (AFC) under physiologic and pathologic conditions. It is unknown whether β1-adrenergic agonists also increase AFC under pathologic conditions. The aim of this study was to investigate the effect of denopamine (β1- adrenergic agonist ) on AFC in hypoxic lung injury and the possible mechanisms involved. Hypoxic rats were exposed to 10% oxygen. 10-5 mol/L denopamine alone or combined with β receptor antagonists, Na+ channel blocker, or Na+/K+-ATPase blocker were perfused into the alveolar space of rats exposed to 10% oxygen for 48 h. AFC and total lung water content (TLW) were measured. AFC did not change for the first 24 h but then decreased after 48h exposure to 10% oxygen. The perfusion of denopamine significantly increased AFC in normoxic and hypoxic rats. The AFC- stimulating effect of denopamine lowered with amiloride (a Na+ channel blocker) and ouabain (a Na+/K+-ATPase inhibitor) by 35 and 53%, respectively. Colchicine significantly inhibited the effect of denopamine. Denopamine can increase the AFC during hypoxic lung injury in rats and accelerate the absorption of pulmonary edema.
Key words: β1-Adrenergic agonist, hypoxia, pulmonary edema, alveolar epithelium.
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