African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12228

Full Length Research Paper

Rapid multiplication of Safed musli (Chlorophytum borivilianum) through shoot proliferation

Nurashikin Kemat1, Mihdzar Abdul Kadir1*, Nur Ashikin Psyquay Abdullah2 and Farshad Ashraf1
1Department of Agriculture Technology, Faculty of Agriculture, University Putra, Malaysia. 2Department of Crop Science, Faculty of Agriculture, University Putra, Malaysia.
Email: [email protected], [email protected]

  •  Accepted: 31 May 2010
  •  Published: 31 July 2010

Abstract

Young shoot buds were used as explants for rapid multiplication of Safed musli (Chlorophytum borivilianum). The explants were cultured onto medium containing basal salts of Murashige and Skoog (MS) and various concentrations of 6-benzylaminopurine (BAP) and kinetin (KIN) for shoot induction. Treatment containing 3.0 mg/l BAP produced the highest mean number of shoots per explants (18.90) and a mean length of shoots (6.0 cm) after 28 days of culture. Regenerated shoots were successfully rooted on MS medium supplemented with 1.0 mg/l indole-3-butyric acid (IBA) and 30 g/l sucrose. For ex vitro establishment, well-rooted plantlets were transferred in potting medium containing vermiculite : organic matters (1:1).

 

Key words: Chlorophytum borivilianum, multiplication, shoot bud, ex vitro rooting.

Abbreviation

BAP, N6-Benzylaminopurine; IBA, indole-3-butyric acid; KIN,kinetin (6-furfurylaminopurine); NAA, α-naphthalene acetic acid; IAA, indoleacetic acid; MS, Murashige and Skoog (1962).