African Journal of

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12300

Full Length Research Paper

The effect of plant growth regulators, explants and cultivars on spinach (Spinacia oleracea L.) tissue culture

Taha Roodbar Shojaei1*, Vahid Salari2, Darioush Ramazan3, Mahdi Ehyaei1, Javad Gharechahi4 and Roya Motallebi Chaleshtori5
1Department of Agronomy and Plant Breeding, College of Agriculture, Islamic Azad University, Karaj Branch, Karaj, Iran. 2Departmet of Agronomy and Plant Breeding, College of Agriculture, Islamic Azad University, Rafsanjan Branch, Rafsanjan. Iran. 3Department of Landscape, College of Agriculture, University of Zabol, Zabol, Iran. 4Department of Agronomy and Plant breeding, College of Agriculture, University of Tehran, Karaj, Iran. 5Departmet of Gene Transformation, Agricultural Biotechnology Research Institute of Iran, Karaj, Iran.
Email: [email protected]

  •  Accepted: 12 March 2010
  •  Published: 31 July 2010


Spinach (Spinacia oleracea L.) is an important vegetable crop of which dioecy in nature has made cultivar improvement difficult using traditional breeding methods; therefore, production of high amount of disease free spinach is critical. To achieve the best explants and media for spinach tissue culture, the effects of two different plant growth regulators, two explants and cultivars on adventitious shoot regeneration were tested. The Analysis of Variance (ANOVA) showed that the effects of plant growth regulators on spinach tissue culture were significant; moreover, the effects of explants were not significant except on the regeneration phase. The best medium for callous induction was MS media containing 1.5 mgl-1IAA + 2.5 mgl-1 GA3. The best medium for shoot regeneration was MS media contained 0.5 mgl-1 NAA + 2 mgl-1 GA3. The best rooting medium was MS medium containing 0.5 mgl-1 IBA. Results presented inhibitory effect of GA3 for callus and root formation; whereas, promote shoot development.


Key words: Plant growth regulators, explants, cultivars, tissue culture, spinach.


MS, Murashige and Skoog; IAA, indole-3-acetic acid; IBA, indol butyric acid; NAA, α –Naphthalene acetic acid; GA3, gibberlic acid; BAP, 6-Benzyl-amino purine; KIN, 6-Furfuryl amino purine; CRD, completely randomized design;SD, short day.