African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12227

Full Length Research Paper

Deletion of the yhdT gene of Bacillus subtilis and its influence on hemolysis

Jie Liu*, Qiong Zhang and Chunhong Fang
College of Life Sciences, Northwest A&F University, Yangling 712100, Shaanxi Province, China.
Email: [email protected]

  •  Accepted: 09 September 2010
  •  Published: 27 September 2010

Abstract

Bacillus subtilis is commonly used as a probiotic. Recently, some metabolites of B. subtilis were reported to cause hemolysis; however, the mechanism by which these metabolites cause hemolysis remains to be clarified. In this study, we cloned the hemolysis-associated gene yhdT and constructed a yhdT gene-deletion mutant of the B. subtilis 224 strain. Further, we determined the hemolytic activity of the culture supernatant of the yhdT gene-deletion mutant and found that the hemolytic activity of the yhdT gene-deletion mutant was lower than that of wild-type B. subtilis224. Thus, our results indicate that the yhdT gene contributes to the hemolytic activity of B. subtilis 224.

 

Key words: Bacillus subtilisyhdT gene, hemolysis.