African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12331

Full Length Research Paper

In vitro daylily (Hemerocallis species) bract multiple shoot induction

Kanyand Matand
  • Kanyand Matand
  • Center for Biotechnology Research and Education, School of Agriculture and Applied Sciences, Langston University, P. O. Box 1730, Langston, OK 73050, USA.
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Meordrick Shoemake
  • Meordrick Shoemake
  • Department of Agriculture and Natural Resources, School of Agriculture and Applied Sciences, Langston University, P. O. Box 1730, Langston, OK 73050, USA.
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Chenxin Li
  • Chenxin Li
  • Department of Plant Biology, College of Biological Sciences, University of California, Davis, CA 95616, USA.
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  •  Received: 21 November 2020
  •  Accepted: 27 January 2021
  •  Published: 28 February 2021

Abstract

A bract is a non-inflorescence structure that exists in many plant species. In daylilies, the bract is biologically functional and is the object of the present tissue culture study. After sterilization, bract explants were treated with 6-benzylaminopurine (BA) and thidiazuron (TDZ) that were used individually or in combination in Murashige and Skoog (MS) nutrients medium, under room environmental conditions, to study its capacity to induce shoots in vitro. The results were successful. Both direct and indirect shoot organogenesis were observed. Although variably, all nineteen cultivars that were investigated induced multiple shoots. TDZ was the most potent chemical stimulus for shoot organogenesis. The results also showed no significant correlation between shoot conversion potential and genotype or treatment.

 

Key words: Daylily, tissue culture, plant organogenesis, bract, Hemerocallis, plant regeneration, thidiazuron.