African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12351

Table of Content: February 2021; 20(2)

February 2021

In vitro daylily (Hemerocallis species) bract multiple shoot induction

A bract is a non-inflorescence structure that exists in many plant species. In daylilies, the bract is biologically functional and is the object of the present tissue culture study. After sterilization, bract explants were treated with 6-benzylaminopurine (BA) and thidiazuron (TDZ) that were used individually or in combination in Murashige and Skoog (MS) nutrients medium, under room environmental conditions, to study...

Author(s): Kanyand Matand, Meordrick Shoemake and Chenxin Li

February 2021

Response of French bean genotypes to Colletotrichum lindemuthianum and evaluation of their resistance using SCAR markers

Common bean anthracnose caused by Colletotrichum lindemuthianum is one of the major biotic constraints to production of French beans (Phaseolus vulgaris L.) in Kenya. This study aimed at screening French bean genotypes in relation to their response to common bean anthracnose in order to identify potential sources of germplasm for breeding. The genotypes were tested in three sites (Kakamega, Mwea and Kutus) where results...

Author(s): Antony Kimani Kamiri, Edith Esther Arunga, Felix Rotich and Reuben Otsyula

February 2021

Analysis of the degradation of polyethylene, polystyrene and polyurethane mediated by three filamentous fungi isolated from the Antarctica

Plastic polymers are petroleum-derived synthetic materials that have multiple uses in everyday life, but their excessive production has led to the accumulation of approximately 1,000 million tons of residues, causing negative ecological impacts. This study analyzed the biological degradation in liquid medium of polyurethane, polystyrene, and polyethylene samples by filamentous fungi isolated from Antarctica. The plastic...

Author(s): Rodrigo Oviedo-Anchundia, Daynet Sosa del Castillo, Jaime Naranjo-Morán, Nora Francois, José Álvarez-Barreto, Alejandro Alarcón, Jhonny Saulo Villafuerte and Milton Barcos-Arias

February 2021

Expression, purification and testing of zinc metalloproteinase aureolysin as potential vaccine candidate against Staphylococcus aureus

Staphylococcus aureus (S. aureus) is a versatile bacterium which exhibits multiple antibiotic resistances. To ameliorate the undesirable diseases causing potential, there is a need to design a protective vaccine capable of stimulating immune response against this pathogen. In a similar study in our laboratory, reverse vaccinology approach was used to nominate potential vaccine candidate genes against S. aureus. Zinc...

Author(s): Samar Solyman, Mohamed Reda, and Amro Hanora

February 2021

A novel genotype-independent technique for successful induction of somatic embryogenesis of adult plants of Jatropha curcas L. using petiole transverse Thin Cell Layer (TCL)

Developing a protocol for somatic embryogenesis (SE) for adult mother plants of Jatropha curcas, aids to overcome problems such as harvesting time and an uneven yield, providing the opportunity to propagate proven elite genotypes. Until now, several authors have achieved SE in J. curcas using different explants, however, in none of these protocols adult plants have been used as mother plant material. Furthermore, the...

Author(s): Cristian Nicolás Mendoza-Peña and Anne Kathrine (Trine) Hvoslef-Eide

February 2021

Identification of zygotic and nucellar seedling of Harumanis mango through molecular markers and morphological approach

Identification of zygotic and nucellar seedlings of Harumanis mango by morphological approach is impossible or hard to be performed. Therefore, this research were to identify zygotic or nucellar seedlings from Harumanis mango polyembryonic seeds through simple sequence repeat (SSR) molecular markers in relation with germination sequence and vigour of seedlings for source of true-to-type cutting material. The...

Author(s): Zul Helmey Mohamad Sabdin, Muhammad Najib Othman Ghani, Shahril Ab Razak, Mashitah Jusoh, Muhamad Hafiz Muhamad Hassan and Ahmad Hafiz Buniamin

February 2021

Aspects of mushroom cultivation to obtain polysaccharides in submerged cultivation

Medicinal and edible mushrooms have biological properties that are important in promoting health. In the last decades, an increasing number of studies have explored various aspects of the cultivation and metabolism of various species. Many isolated substances have potential for use in medicine and among them are polysaccharides, which have been shown to have physiological properties such as anti-tumor, immunomodulatory,...

Author(s): L. H. Campestrini and Salles-Campos C.